Based on the perception anticipated by chan.et.al.,  alkali metal cations (Na+) could rapidly intercalated / de-intercalated into the bulk of MnO2, as soon as the electrode was dipped into the electrolyte (1 M Na2SO4
), so the reversible redox reactions could be represented as;
Afterward, about 200 mL aqueous Na2SO4
(2.5 g per 100 mL) and dichloromethane (25 mL) were sequentially added to the above separatory funnel followed by vigorous shaking for two minutes.
All rejection tests were performed under 50 psi (0.34 MPa) and the rejection tests in (a) were performed with 20 mM NaCl, 10 mM Na2SO4
, 7.5 mg/LMB, and 7.5 mg/L R-WT solutions, respectively.
Categorized sample is dipped into the solution of Na2SO4
(specific gravity of 1.71 to 1.54) for eighteen hours.
The reaction was quite clean which afforded succinimde as the only side product which was filtered off and the organic layer after washing with water and drying over anhydrous Na2SO4
afforded the 2-bromoindanone 14 as colorless solid.
The pH of the hemolysate was adjusted to 8.7 with a solid Tris base, and applied to the prepared Sepharose 4B-L-tyrosine-sulfonamide affinity column equilibrated with 25 mM Tris-HCl/22 mM Na2SO4
Nitrogen fixing characteristics were investigated by observing the growth of endophytic bacterial isolate on a nitrogen-free solid agar, Burk's N-free medium, containing (per liter) 10 g glucose, 0.41 g KH2PO4, 0.52 g Na2SO4
, 0.2 g CaCl2, 0.1 g MgSO4.7H2O, 0.005 g FeSO4.7H2O, 0.0025 g Na2MoO4.2H2O and 15 g agar, pH 7+-0.1 (Wilson and Knight, 1947).
The water was removed from this layer using anhydrous Na2SO4
. After the ethyl acetate layer was evaporated, the remaining ester was colorless.
The essential oils were dried over anhydrous sodium sulfate (Na2SO4
After vortex mixing, 0.5 mL Tris/HCl buffer (50 mM; pH 7.4) was added followed by further mixing and incubation at room temperature for 10 minutes; 1.0 mL of 0.75% thiobarbituric acid in 2 M Na2SO4
was added and then the mixture was heated at 100[degrees]C for 45 minutes.
The water phase was extracted 3 times with EA; the combined organic solutions were washed with brine, dried over anhydrous Na2SO4
, and concentrated using a rotatory evaporator.
Acrescentou-se sulfato de sodio (Na2SO4
) na amostra recolhida, garantindo assim a retirada da agua e outras impurezas.