For the 1st experiment, 50 g seeds of each wheat cultivars were soaked in aerated solution of 1.0, 0.5, 0.1, 0.05 and 0.01 M B [boric acid (H3BO3), B = 17% (MERCK, Purity = 99.5%) and borax (Na2B4O7
), B = 11% (MERCK, Purity = 98%)] or water (hydropriming) for 12 h at 25 +- 2degC and then dried back to their original weight.
Induced resistance of rice plant to blast (Pyricularia grisea) by seed treatment using natri tetraborate (Na2B4O7
) under field condition.
The screening of isolated bacterial strains for PHA production was done by growing them on PHA-detection agar (PDA) medium [(NH4)2SO4, 2 g/L; KH2PO4, 13.3 g/L; MgSO4.7H2O, 1.3 g/L; citric acid, 1.7 g/L; agar, 15 g/L; glucose, 2 g/L; trace element solution, 10 mL/L (FeSO4.7H2O, 10 g/L; ZnSO4.7H2O, 2.25 g/L; CuSO4.5H2O, 1 g/L; MnSO4.5H2O, 0.5 g/L; CaCl2.2H2O, 2.0 g/L; Na2B4O7
.10H2O, 0.23 g/L; (NH4)6MO7O24, 0.1 g/L; HCl,10 mL)] (Lee and Choi, 1999).
The refined products are Sodium tetra borate, (Na2B4O7
;5H2O) borax (Na2B4O7
;10H2O), sodium penta-borate (Na2B10O16;10H2O), solubor (Na2B8O13;4H2O) and boric acid (H3BO3).
The buffer solutions in the pH range 1-12 at an unit interval of one pH unit were prepared from KCl (1M) adjusted with HCl (0.1M) at pH 1 and 2, CH3COOH (1M) and CH3COONa (1M) (pH 3-6), CH3COONH4 - NH3 pH 7, H3BO3 - Na2B4O7
(1 M) at pH 8-9, Na2CO3 (saturated) NaHCO3 (I M) pH 9-10, NH3 (1M) NH4Cl (1 M) 10-11 and NaOH(1M) NaCl (1M) pH 12.