Characterisation of two deletions involving NPC1
and flanking genes in Niemann-Pick type C disease patients.
In 2011, Chandran, of Albert Einstein College of Medicine in New York City, and others identified NPC1 as Ebola's potential partner in crime (SN Online: 8/24/11).
But scientists didn't know just how crucial NPC1 was for Ebola's dirty work.
If it cannot access NPC1, it loses the ability to infect and kill its victims.
Dr Kartik Chandran, of the Albert Einstein College of Medicine in New York, said: "Mice lacking both copies of the NPC1 gene, and therefore devoid of NPC1 protein, were completely resistant to infection."
Together, these studies suggest that cells and tissues with high NPC1
and TIM-1 expression may be major sites of viral infection and sites of significant shedding of mature infectious viral progeny.
Within the cholesterol metabolism, both miR-33 and miR-33* target ATP-binding cassette A1 (ABCA1) and Niemann-Pick disease C1 (NPC1
), that is, molecules important for loading cholesterol into HDL particles and for cholesterol transport from the lysosomal compartment within the cell, respectively [6, 7, 9, 10].
One of the mechanisms regulating cellular cholesterol homeostasis is export from endosomes by Niemann-Pick disease proteins (NPC1
and NPC2) (Cheruku et al, 2006).
One such cellular protein, known as Niemann-Pick C1 (NPC1
), stood out.
For cyclic compressive loading, the test was conducted on NPC1
To analyze these force recordings, we evaluated a variety of characteristics: maximal force (Fmax), mean force during the first minute of stimulation (Fm1n), number of series of claps (Nps), number of tonic contractions (Ntc), number of claps per series of claps (Npc1
, Npc2, Npc3 etc) mean force change per series of claps (Fpcm1, Fpcm2, Fpcm3 etc), clapping rate during each series (rpc1, rpc2, rpc3 etc), force change and duration of the tonic contractions (Ftc1, Ftc2, Ftc3, etc and Dtc1, Dtc2, Dtc3 etc, respectively), maximum number of claps per series (Npc max), mean clapping rate (rpcm) and total number of claps(Npctot).
The second variant linked to child and adult obesity is found in the NPC1
This identity was confirmed by ProteinChip immunoaffinity capture assay with antiITIH-4 antibody, which specifically captured the 3953 Da peptide in serum fractions of 3 NPC patients (NPC1
, 2 and 3) but not or in only very small quantity in the 3 control serum fractions (Fig.