This is likely due to the introduction of blood to cerebrospinal fluid (CSF) as the number of myeloblasts
found in CSF has been correlated with a worse prognosis [19-21].
and megakaryocytes were normal in number and morphology; M : E ratio was 6: 1.
The WBC precursor channel (WPC), one of the new channels, is based on fluorescence flow cytometry technology and was developed particularly to detect myeloblasts
or lymphoblasts more accurately than the immature myeloid information channel used in the XE series.
It is not unusual to identify two distinct blast populations in the same patient, one of small size with a high nucleus/cytoplasm ratio resembling lymphoblasts and the other larger with more abundant cytoplasm with or without granulation resembling myeloblasts
Gate D (pink) is shown here in the area typically occupied by myeloblasts
, but may be used to highlight other populations.
In more than 90% of patients suffering from acute myeloid leukaemia, CD33 has been recognised to be the antigen expressed on the surface of myeloblasts
. By binding to the CD33 antigen on the cell surface, Mylotarg is absorbed into the cell and calicheamicin is released, causing death to the cancer cell.
Liver showed a massive intra and extra vascular accumulation of myeloblasts
, and showed congestion in the portal vein with focal lymphoid cells (Fig.
MYLOTARG is an antibody-drug conjugate (ADC) composed of the cytotoxic agent calicheamicin, attached to a monoclonal antibody (mAB) targeting CD33, an antigen expressed on the surface of myeloblasts
in up to 90 percent of AML patients.
Fluorescence in situ hybridization confirmed the CBFp rearrangement associated with inv(16) in myeloblasts
and myelomonocytic cells but not in CLL cells, confirming that the AML and CLL did not share clonality.
Bone marrow cells were collected and performed the following examinations: (a) cytology showed marked hypercellular marrow with 2.5% myeloblasts
, 4.5% promyelocyte, 31.5% myelocyte, 14.5% metamyelocytes, increased number of megakaryocytes, and neutrophil alkaline phosphatase score at 30 (normal range 13–130); (b) cytogenetic analysis revealed 46, XY, t(9;22)(q34; q11.2) in 10 of 10 metaphase cells; (c) molecular analysis by quantitative polymerase chain reaction found P190 positive with negative results for P210 and P230.
RTD1 RTD2-3 RTD4-6 References Monocytes, Monocytes, Neutrophils, [29, 31, 109, neutrophils, neutrophils, myeloblasts
135, 136] myeloblasts
Bone marrow biopsy and aspirate showed 73% myeloblasts
and immature promonocytes expressing CD13, CD15, CD33, CD45, CD64, CD123, and HLA-DR by immunohistochemistry (IHC) and flow cytometry (FC).
Moloney, "Chloroma and other myeloblasts
tumors," Blood, vol.
Bone destruction was attributed to the invasion of leukemic myeloblasts
. However, the mechanism, by which ML cells produce destructive bone lesions, and the effect of therapeutic intervention on osteolytic lesions during ML treatment are not fully understood.
Histologic examination of the spleen indicated a massive intravascular and extravascular accumulation of myeloblasts
with a variable proportion of promyelocytes and myelocytes in the red pulp of the spleen.