CYP3A4*1G genetic polymorphism influences metabolism of fentanyl in human liver microsomes
in Chinese patients.
Effect of hyperthyroidism on the in vitro metabolism and covalent binding of 1, 1-dichloroethylene in rat liver microsomes
. J Toxicol Environ Health 1997;52:169-88.
For each substrate, we prepared at least eight different substrate concentrations in methanol (Table 1) and further diluted them in the reaction mixture to a final volume of 100 [micro]L, containing 50 mM phosphate buffer at pH 7.4, 10 mM magnesium chloride, the specific concentration of liver or intestinal microsomes
(0.1 mg/mL for BPS, 0.05 mg/mL for BPF, and 0.01 mg/mL for BPAF), and alamethicin (5% of the total protein concentration).
Genistein showed notable inhibitory effects on human (34%) and rat liver microsomes
(38%) in vitro.
In vitro stability tests against mouse liver homogenates (S9 fraction) and purified mouse liver microsomes
showed 66.9 [+ or -] 6.7% and 31.4 [+ or -] 7.8% intact tracer after 120 min, respectively.
For Western blotting, microsomes
(5 [micro]g) were size-separated by 10% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes.
Zhu et al., "Metabolism of dictamnine in liver microsomes
from mouse, rat, dog, monkey, and human," Journal of Pharmaceutical and Biomedical Analysis, vol.
To confirm this result, alantolactone and isoalantolactone each was incubated in vitro with gastric juice, intestinal juice, fecal bacteria, and liver microsomes
. The lactones were found to be stable in gastric juice, intestinal juice, and fecal bacteria, respectively.
Incubation of NPS with prepared human liver microsomes
and identification human urinary metabolites by mass spectrometry is the most cost-effective technique of several available approaches.
The livers were removed immediately and washed in ice-cold water and the microsomes
were prepared, as described previously.
(13) showed that ritonavir is metabolized by CYP 3A4 and CYP 3A5 at the same ratio, but indinavir is metabolized by CYP 3A4 than CYP 3A5 in human intestinal microsomes
and expressed CYP 3A4/3A5.
The liver microsomes
to be used in the assay for HMGCR activity were prepared as described by Honda et al.
Weinz, "In vitro metabolism of rivaroxaban, an oral, direct factor xa inhibitor, in liver microsomes
and hepatocytes of rats, dogs, and humans," Drug Metabolism and Disposition, vol.
Venkatakrishnan et al., "O- and N-demethylation of venlafaxine in vitro by human liver microsomes
and by microsomes
from cDNA-transfected cells: Effect of metabolic inhibitors and SSRI antidepressants," Neuropsychopharmacology, vol.
Mitochondria and microsomes
from freshly extracted mouse liver were prepared by differential centrifugation and suspended in 20 mM [K.sub.2]HP[O.sub.4] buffer containing 20% glycerol with added leupeptin, pepstatin, antipain, and PMSF as described previously [29, 30].