VGS have been long recognized as difficult to identify at the species level using conventional microbiological techniques
, and even with 16S rRNA sequencing.
General microbiological techniques
. In: Standard operating procedure manual for microbiology laboratories, National Institute of Biologicals, Ministry of Health and Family Welfare, Government of India.
The surviving bacteria were enumerated using standard microbiological techniques
for determining the D-10 value.
A total number of 17,651 samples including blood, urine, CSF, pus and catheter tips from suspected cases of bacterial infections were processed and test organisms were identified using standardized microbiological techniques
. MBL phenotypic identification was performed by Modified Hodge Test, Double Disc Synergy Test and Combined Disc Test.
The genomic analysis also revealed that using traditional microbiological techniques
such as serotyping to understand the spread and diversity of the bacteria is unhelpful for planning public health campaigns and for creating effective vaccines.
The problem is further compounded as experienced laboratorians are retiring, taking with them years of valuable experience running and interpreting traditional microbiological techniques
. These trends, along with remaining lab professionals being stretched by continual cost-cutting measures, make the laboratory environment increasingly stressful and have helped fuel the demand for better diagnostics to help uncover the etiological agents behind patient illnesses.
Each unique colony of isolated microorganisms was identified, and their antimicrobial susceptibility was tested using standard microbiological techniques
. Positive tissue cultures were considered those with the same microorganism isolation of at least two periprosthetic tissue samples.
Recent advances in the better understanding of how organisms behave and of microbiological techniques
such as sonification of the biofilm around the implant and prolonged culture have produced numerous reports showing that in cases labelled aseptic loosening, organisms of low virulence such as Propionibacterium acnes (a skin commensal) can be identified in up to 72% of cases.
The samples were processed according to standard microbiological techniques