Biochemical analysis of the genus Bacillus gave negative results for indole, citrate, oxidase, and urease and positive results for methyl red
Voges-Proskaeur, catalase, and gelatinase tests.
Because Cy3 and nitro methyl red
are able to combine in the probe, quenching fluorescence, without the need for self-complementary pairing, "this strategy will be applicable to the design of peptide-based probes," conclude the researchers in their paper published in the journal Science and Technology of Advanced Materials.
curvatus) characterised through molecular characterization was found to be negative for catalase test, indole test (green layer is formed at the top of test tube), nitrate reduction test, citrate test (no blue colour in test tube), methyl red
Voges-Proskeur test (no red colour appears in test tube), casein hydrolysis test (not able to produce protease enzyme) and for starch agar test (not able to produce amylase enzyme) (Table 1).
Usability of aqueous solutions of methyl red
as high-dose dosimeter for gamma radiation.
The distillate was collected for 3 min into 10 ml boric acid (20 g/L) containing five-six drops of mixed indicator (2 g/L methyl red
indicator alcohol solution and 1 g/L methylene blue indicator aqueous solution).
All strains were found to be negative for methyl red
and Voges-Proskauer, indole test, gelatin liquefaction, starch hydrolysis, and [H.sub.2]S production, except for SK01 and SK04 strains, which tested positive for gelatin liquefaction, and SK07 positive for methyl red
The results of morphological, physiological and biochemical analyses showed that the isolates were Gram positive, motile, and positive for catalase, nitrate, citrate, methyl red
(MR), lysine decarboxylase, ornithine decarboxylase, methyl red
(MR) and Ortho-Nitrophenyl-Beta-D-Galactosidase (ONPG) tests, but were negative for oxidase, urease, indole, H2S production, arginine dihydrolase, phenylalanine and tryptophan deaminase and Voges-Proskauer (VP) tests.
The results obtained by using conventional biochemical tests confirmed the significant difficulties for Klebsiella differentiation at the species level, even when reference strains were included and biotyping by other methods; some inconsistent findings were obtained for Methyl red
and Voges Proskauer reactions.
The following chemicals were used: Potassium bromate (Kbr[O.sub.3]), ACS reagent; SRM 83d, Arsenic Trioxide ([As.sub.2][O.sub.3]); 5 mol/L sodium hydroxide (NaOH) prepared from analytical reagent grade; 10 mol/L high-purity hydrochloric acid (HCI); and 1 % (mass fraction) methyl red
indicator in ethanol (200 proof).
Basfar, Radiation-induced color bleaching of methyl red
in polyvinyl butyral film dosimeter, Radiat.
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indicator,Methyl red indicator
oxidase, catalase, urea and IMViC pattern (indole production, Methyl Red
(MR) test, Voges-Proskauer (V.P.) test, citrate utilization on Simmon's citrate medium).
Biochemical characterization of the bacterial isolates was done by catalase test, methyl red
test, Oxidase test, Indole test, Voges proskauer test, DNAase test, Sudan III test and Motility test using Sulfide Indole Motility agar (Tittsler and Sandholzer, 1936).
The positive colonies were subjected to biochemical test for identification such as catalase, coagulase, oxidase, indole, methyl red
, Voges-Proskauer, Citrate, TSI, stormy clot fermentation, Lecithinase, CAMP tests and sugar fermentation reaction as per Barrow and Feltham (1993).
Gram negative colonies that grew on this medium were subjected to biochemical tests (Indole and oxidase production tests, methyl red
test, Voges Proskauer test and citrate utilization test) to confirm the colonies as E.