Streptococci were initially identified using CAMP test, esculin hydrolysis, hemolysis, and no growth on MacConkey agar
. Isolated Streptococci were further identified using Strepto system 9R (Lio Filchem S.KI, Italy).
Non-typical characteristics of the isolates obtained in the present study included no growth in MacConkey agar
, as well as beta-hemolytic negative colonies and positive beta-galactosidase ONPG, which is atypical for M.
All clinical isolates were cultured on blood and MacConkey agar
and then tested for Gram staining to identify Gram reaction and bacterial morphology.
All specimens were inoculated on blood and macConkey agar
incubated aerobically at 35AoC for 18 to 24 hours.
The TSA, blood agar, and MacConkey agar
plates were incubated aerobically at 35[degrees]C, and the Sabouraud agar plates were incubated at 25[degrees]C.
All the specimens were inoculated on the MacConkey agar
medium containing 2mg/L cefotaxime.
CHROMagar[TM] ESBL and MacConkey agar
with a 10 [micro]g meropenem disc.
They appeared as non-lactose fermenters in the MacConkey agar
with positive oxidase reaction.
Further, the stool specimen was plated onto the Blood and MacConkey agar
plates (HiMedia Laboratories, Mumbai, India) and incubated aerobically at 37[degrees]C for 24 hours.
Blood agar (BA) and MacConkey agar
(MCA) were used as primary culture media for preliminary isolation of organisms from samples according to methods described by Cruickshank et al.
Out of each sample, 25 g were inoculated into 250 ml peptone water (enrichment broth), incubated at 37degC for 24 h and then, cultivated onto nutrient agar, eosin methylene bile agar (Kim and Bhunia, 2008) and MacConkey agar
media and incubated for 24 h at 37degC.
These samples were divided into 3 groups and inoculated into MacConkey agar
and Eosin Methylene Blue agar after that the results were noted (1-2).
All the samples were processed and inoculated on blood agar, nutrient agar, and MacConkey agar
The biological profiles were checked by using MacConkey agar
, Nutrient agar and Lauryl Tryptose broth (Carolina Biological Supply, Inc.).
The swab was used to inoculate the center area of 2 Fisher Brand Sterile 100 mm x 15 mm Polystyrene Petri dishes containing Mannitol Salt agar (Carolina Biological Supply Company, Burlington, NC) and MacConkey agar
(Baltimore Biological, Baltimore, MD).