Heterochromatin in yeast forms at the telomeres and propagates centromerically for about 3-4 kilobases
Table 1 Fragments per kilobase
per million reads (FPKM) values reveal that opsin transcripts are abundant in the cerebral ganglion (CG) and are lowly expressed or absent in the subesophageal ganglion (SEG), thoracic ganglia (T7-9), and abdominal ganglia (A1-6) Opsin sequence CG SEG T7-9, A1-6 NoTranscript20086 95.
The mean number of SNPs per kilobase
in the ORF region was 12,691.
These primers could also detect variant 2, which would yield a considerably longer amplicon (approximately 1 kilobase
[kb]): The forward primer Fusion-RT-S, located in exon 13 of EML4, is 5'-GTG CAG TGT TTA GCA TTC TTG GGG-3', whereas the reverse primer Fusion-RT-AS, in exon 20 of ALK is 5'-TCT TGC CAG CAA AGC ACT AGT TGG-3'.
To compare miRNA concentrations across the data set, we corrected the sequencing bias of GC content for each miRNA (3) and normalized the sequencing read count as reads per kilobase
of exon model per million mapped reads (RPKM) (4).
These genome-wide technologies use in situ hybridization for SNP detection and contain anywhere from 1,000 to 500,000 SNP markers, with about one marker per five kilobase
, or 5 kb.
pairs (kb) according to the independent restriction fragment profiles.
The system targets promoters, canonical CpG islands, and the more recently described "shores" and "shelves" found up to 4 kilobase
pairs on either side of CpG islands.
2016), we calculated the expression levels of the candidate genes in fragments per kilobase
of exon per million fragments mapped (FPKM) using the "built in" option of the Trinity assembly package (Grabherr et al.
A 3D map of the human genome at kilobase
resolution reveals principles of chromatin looping.
Gene expression level analysis was performed by the reads per kilobase
per million mapped reads (RPKM) method using the formula RPKM = 103C/NL, where C is the number of mappable reads that uniquely align to a unigene, N is the total number of mappable reads that uniquely align to all unigenes, and L is the sum of the unigenes in base pairs (Mortazavi et al.
Levels of gene expression were normalized using values of the fragments per kilobase
of exon per million mapped reads (FPKM).
The resulting modified raw gene count was then divided by the total number of uniquely aligned reads for the sample, yielding reads per kilobase
per million uniquely mapped reads (RPKM) (15).
All breaks in MLL occur within the same 8 kilobase
(kb) region, (bcr) and create in-frame fusion mRNAs and fusion proteins with the translocation partner gene.