These Candida-positive colonies were gram stained and only those which were round to oval with purple-coloured budding yeast cells were further processed for germ tube
album was most effective to inhibit spore germination (48%) and germ tube
In present study, herbal toothpast 4 was further assayed for its inhibition activity on germ tube
formation, or mycelial conversion, and oral epithelium adhesion of C.
Out of 87 isolates, 27 isolates were positive for germ tube
test and growth at 45[degrees]C on SDA media indicating all 27 isolates were C.
To confirm the presence of Candida, Gram staining and germ tube
test were performed.
Speciation of Candida isolates was done by the germ tube
test, morphology on corn meal agar and Chromagar.
All species which were positive for germ tube
and chlamydospore production were cultured on SDA and kept at 45C for 48-72 h.
albicans ATCC10231 provided by American Type Culture Collection (ATCC), Rockville, MD, USA, was employed for determining the antifungal PDI (APDI), fungicidal studies and germ tube
Culture filtrates of these isolates were tested to determine their potency to inhibit germination and decrease germ tube
length of conidia of the test pathogen.
M, we observed the growth and branching of the hyphae from the germ tube
i, through the formation of the germ tube
or appressoria, similar to the findings obtained with C.
Until then it had been misidentified as candida albicans if only germ tube
test was used for identification as both the species have many similarities in their microscopic morphology and ability to form germ tubes
The fungus was identified by demonstrating formation of a germ tube
on 4-hour incubation in horse serum.
It was clear that the vigor and growth of the germ tube
in all of the pathogens tested was markedly reduced even at the lowest concentration tested.
It is believed that chlorhexidine inhibits germ tube
formation in Candida albicans there by suppressing the candidal activity .