Next, the whole content of each well was transferred to a sterile tube containing 100 [micro]l of human serum as inducer of germ tube formation.
To corroborate the killing action of [alpha]-T, aliquots obtained from viable cells reduction experiments at different times (see Section "Inhibition of germ tube formation") were stained with 10 [micro]l of aqueous propidium iodide (PI) (Molecular Probes, Eugene, USA) solution (10 [micro]g/ml) and fluorescein diacetate (FDA) (Sigma-Aldrich, St.
clarum in relation to the emergence of the germ tube
and the process of spore formation were similar to those described by Maia et al.
Initial studies compared the use of germ tubes
of germinating spores, and ungerminated spores, as sources of sphaeroplasts.
6] germ tube
cells emulsified in Freund's complete adjuvant was injected subcutaneously into an egg-laying hen.
Strains showing ability to induce germ tubes
in Lee's simplified media (LSM) at pH 6.
In addition to germ tube
inhibition, budding was also inhibited considerably at sub-inhibitory concentrations (Fig.
Flow cytometry Germ tube
Cell cycle Synergism Amphotericin B Checkerboard method
Linalool, citral, citronellal and benzyl benzoate caused more than 50% inhibition of germ tube
induction at 0.
Subculture on ChromAgar (ChromAgar Candida, Paris, France) grew apple-green colonies, which were germ tube
After incubation, a drop of the culture was observed under a microscope for germ tube
formation and compared with the control (without drug) (Odds, 1988).
All yeast isolates were initially identified by germ tube
and chlamydospore formation as C.
In this test Candida albicans would be reported, if the germ tubes
were observed and if not, the germ tubes
of medium candida species were used for detection.
albicans (producing germ tubes
and chlamydospores) suggest that some C.