GzmB increased in NK cells after induction of peritonitis in both PLF and blood, expressed either as percentage of positive cells and MFI (Figures 2(c) and 2(d)).
With the aim of evaluating whether gzmA and/or gzmB deficiency influences local bacterial outgrowth and subsequent dissemination, we determined bacterial loads in the PLF, blood, and liver and lung homogenates of WT, [gzmA.sup.-/-], [gzmB.sup.-/-], and [gzmAxB.sup.-/-] mice at predefined time points after infection (Figure 3).
To get insight into the influence of gzmA and gzmB deficiency on the local inflammatory response elicited during septic peritonitis, we measured proinflammatory (TNF-[alpha], IFN-[gamma], and IL-1[beta], IL-6) and anti-inflammatory (IL-10) cytokines as well as MCP-1 and KC levels in PLF.
As Bregs are defined by a complex phenotype that include simultaneous positivity for the markers CD19, CD38, CD5, GZMB, and IL10 , cells with this phenotype were investigated using multiparametric flow cytometry on the viable single-tumour cell suspension obtained from a fresh tumoural sample of patient no.
This Th1 signature, which was identified by means of positive staining for GZMB, Tbet, and MUM-1, and the presence of [CD8.sup.+] cells expressing nuclear EZH2, was switched off in Pro-E-MpMs, HG-E-MpMs, and in S-MpMs.
(a) Representative IHC images of CD3, Tbet, and granzyme B (GZMB) in an E-MpM (no.
The expression of activation or functional markers by [CD8.sup.+]T cells was measured by flow cytometry, directly for CD25, CD107a, and CD69 or after an intracellular staining protocol for IFN-[gamma] and granzyme B (GZMB