Given alternative sources of stem cells, including nonembryonic sources that show promise in their own right, how shall we weigh claims about the relative pace of progress against the moral offense to many citizens in publicly funding embryo or ES cell
research, even if the latter is believed to have somewhat greater efficacy?
Research using human ES cells
could help to better understand early human development, be used to research possible toxic effects of drugs (drug-screening) and, most importantly, be used in the field of regenerative medicine in the development of cell replacement therapies.
Such breakthrough stem cell technology has presented human ES cell
therapy derivatives as a powerful pharmacologic agent of cellular entity for a wide range of incurable or hitherto untreatable neurodegenerative and heart diseases.
The trials are privately funded by biotechnology companies in the USA, and do not involve injection of ES cells
but rather the use of ES cell
lines that have been induced to differentiate into particular cell types.
Briefly, ES cells
were cultured in ES cell
medium without the feeder layers for 24-28 h, then the clumps were mechanically separated and cultured in suspension in LIF-free medium containing 15% FBS for 5-10 days until EBs formed.
The potential of mouse ES cells
to generate functional DA neurons and to correct behavioural deficits after engraftment into Parkinsonian rats has been demonstrated (7,8).
Under the service agreement, SCS will create standard culture conditions for CHDI's mouse ES cell
lines using its serum-free and feeder-free ESGRO Complete media.
Mouse ES cells
were grown in vitro and ES cells
injected into mice which formed teratomas.
Researchers at Children's Hospital of Boston and the Harvard Stem Cell Institute together with an international set of collaborators completed a thorough genome-wide analysis of five novel pES cells, 10 mouse ntES, as well as the human ES cell
line purportedly generated by Hwang and colleagues using human eggs and somatic nuclear transfer.
However, accepting that we can now manipulate ES cell
lines to differentiate into practically any tissue of choice, there remain substantial obstacles before the therapeutic potential of ES cells
can be exploited.
The Thomson experiments simultaneously announced a scientific triumph and a bioethical dilemma, however, because human embryos (obtained, in this case, with the consent of donors from several in vitro fertilization clinics) had to be destroyed in order to start the ES cell
President Bush and his supporters among religious conservative groups claim that ES cell
research constitutes murder of developing life, while others--scientists, patient advocates, and politicians across the spectrum--view ES cells
as potential cures for many diseases (Bush, 2001, 2005; Daley, 2004; Wade, 2003).
Based on the analysis results, the company will compile and commercialize an ES cell
library of gene knockout mice with a view to offering it to pharmaceutical companies and research institutions around Japan.
The insights into development processes and disorders that ES cell
research would provide would be profoundly beneficial.
RAX230) ROBERT KLUPACS - ES CELL
INTERNATIONAL PTE LTD.