coli strains positive for STa and/or F5 were classified as ETEC; if positive for Stx2 and/ or Stx1,they were considered STEC; EHEC
strains were identified as positive for Stx and intimin; strains positive for intimin only were classified as EPEC; strains positive for CNF2 were classified as NTEC; Strains positive for EAST-1 only were classified as EAEC pathovars.
List of various bacterial strains employed to determine specificity of IgY by dot-ELISA Bacterial strain Result EPEC *USFDA-303 + EHEC
*ATCC 43893 + EIEC *ATCC 43887 + ETEC *ATCC 35401 + EAEC *DFRL- SC-542 + Bacillus subtilis * ATCC-6633 - Salmonella typhimurium DRDE-GWALIOR - Shigella spp.
jejuni: 90%, Salmonella: 40%, EHEC
: 39%), and gram-negative bacteria were used as the pathogen surrogate.
For infection, EHEC
derivatives were cultured in LB at 37[degrees]C for 16 h with appropriate antibiotics.
coli included five outbreak strains of different serotypes with varying stx-profiles, of which four strains were EHEC
(Table 1), also used in a previous study by Rode et al.
Final presentation and evaluation of epidemiological findings in the EHEC
O104:H+ outbreak Germany 2011.
Originally developed for isolating EPEC serotypes this medium is recommended for the isolation and differentiation of EHEC
Food contamination has been identified as a potential source of pathogenic EHEC
O157 transmission in humans [8, 9].
htm) realizandose las etapas de enriquecimiento y siembra, la primera con caldo EHEC
suplementado con cefixime, cefsulodin y vancomicina y la segunda en Agar Mc Conkey sorbitol con cefixime y telurito.
Either toxin detection with any of several immunoassays or detection of the shiga toxin genes with molecular methods will provide superior detection of the diverse range of EHEC
Recognised classes of Escherichia coli (EPEC, ETEC, EHEC
, EAEC, and EIEC) were not isolated due to lack of facility in the institution.
The most common cause of (D+) HUS is enterohaemorrhagic Escherichia coli (EHEC
), serotype 0157:H7.
) may cause haemorrhagic colitis (HC) and haemolytic uremic syndrome (HUS) owing to the production of shiga toxins (Smet et al., 2011).
EF-P plays a crucial role in the production of proteins that are essential for the virulence of EHEC
Figure 2 shows an amplification plot demonstrating the presence of stx stx and eae target genes with the foodproof EHEC
screening kit in a culture of E.coli 0157, H7.