The oocytes injected with Egr3 siRNA or Xpress-[DELTA]Egr3-DsRed2 RNA were fixed in 3.7% formaldehyde in phosphate-buffered saline (PBS) and permeabilized in 0.25% triton X-100 in PBS.
293T cells were co-transfected with pcDNA3.1/Myc-Egr3 plasmid and Egr3 siRNA.
To examine if Egr3 is expressed in mouse gonads at different stages of sexual maturation, we collected ovaries and testes from 1, 2, 3, and 4 week-old mice.
We previously showed that the immunoreactive Egr3 is specifically localized near MTOCs and on spindle during meiotic maturation of oocytes .
Meiotic maturation is not hindered by Egr3 siRNA in oocytes
We next tested if microinjection of Egr3 siRNA has any influence on meiotic maturation.
The results reported here demonstrate that Egr3 selectively regulates the DG-specific perdurance of Arc transcription.
The current data indicate that Egr3 is a viable target for such a manipulation to decipher the specific contribution of late-phase Arc transcription to hippocampus-dependent learning and memory in intact animals.
Milbrandt, "Sensory ataxia and muscle spindle agenesis in mice lacking the transcription factor Egr3," Nature Genetics, vol.