DNA

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Moreover, the fluorescence intensities of the mixture were weak in the absence of target HIV-1 dsDNA (curve b), capture DNA (curve c), or signal DNA (curve d).
In this study, the techniques of volume and surface integration are used to compute the total interaction energy of the ssDNA and dsDNA molecules with a lipid bilayer.
Effect of dsDNA binding to SmD-derived peptides on clinical accuracy in the diagnosis of systemic lupus erythematosus.
One study found proliferative LN to be significantly correlated with haematuria on dipstick (p < 0.0001), proteinuria on dipstick (p = 0.042), low complement C3 (p < 0.0001), low complement C4 (p = 0.009) and positive dsDNA (p = 0.039).
The DNA SMART ChIP-Seq Kit makes it possible to further expand the benefits of SMART technology into other NGS applications, allowing the company to address the needs of those researchers working with low inputs of either dsDNA or ssDNA for NGS applications, Lou added.
Upon exposure to anti-ribonucleoprotein antibodies, these neutrophils release extracellular traps, which contain dsDNA and other proteins that ultimately stimulate type I interferon production by plasmacytoid dendritic cells [23].
Total soil dsDNA was extracted in duplicate from dried soil ground to <250 [micro]m with pestle and mortar.
The production of these autoantibodies could be antigenic driven, the result of polyclonal B-cell activation, impaired apoptotic pathways or becausedifferent autoantibodies [ANA (Orgentec 538), dsDNA (Orgentec 604), SSA (Orgentec 508), SSB (Orgentec 509), anti-histone (Orgentec 507), Sm (Orgentec 510)] by an ELISA assay.
[6] could not find any correlation between ribosomal P antibodies and lupus nephritis while Reichlin and Marianne [7] found a strong association of anti dsDNA and of anti ribosomal P antibodies with lupus nephritis.
In addition to these strand-break substrates, we determined the effects of 3 on the structure and stability of dsDNA, fork, 3' and 5'-OvHg.
Binding to dsDNA produces a ~20-fold fluorescence enhancement, emits blue fluorescence upon excitation with mercury-arc lamp or UV lines of the argon-ion laser (Ex/Em = 358/461 nm).
Specific Parameters of Systemic Lupus Erythematosus Study Group Parameter Mean 6 SD Anti-dsDNA antibodies 160 [+ or -] 40.5 C3, mg/dL 68.91 [+ or -] 11.91 CRP, mg/dL 180 [+ or -] 20.5 SLEDAI 12.66 [+ or -] 3.70 Abbreviations: C3, complement; CRP, C-reactive protein; dsDNA, double-stranded DNA; SLEDAI, Systemic Lupus Erythematosus Disease Activity Index.
These repeat sequences serve as the detection sites by hybridizing with detection probe to form double strand DNA (dsDNA) on the long ssDNA.
The dynamic range allows the analysis of dsDNA up to 8000 ng/pL without physical dilution.