RNA interference in the light brown apple moth, Epiphyas spostvittana (Walker) induced by double-stranded RNA
"There are insects that just laugh at naked double-stranded RNA
," he says.
(1974) "Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA
. Nucleic Acids Res.
Detection and characterisation of bisegmented double-stranded RNA
viruses (Picobirnavirus) in human faecal specimens.
Riboxx Pharmaceuticals, a company focusing on the development of agonists of Toll-like Receptors, with a direct application as modulators of innate immunity, has received a patent for the process of manufacturing double-stranded RNA
(dsRNA) for increased stability in biological fluids from the European Patent Office, it was reported yesterday.
Abbreviations DBP: Albumin D site-binding protein dsRNA: Double-stranded RNA
HLF: Hepatic leukemia factor PAR: Proline- and acidic amino acid rich PDP1: PAR-domain protein 1 RNAi: RNA interference SGADs: Second generation antipsychotic drugs TEF: Thyrotroph embryonic factor.
Dicer is a critical enzyme involved in the RNAi gene silencing cascade and acts earlier in the pathway, preparing double-stranded RNA
RNA interference involves introducing long double-stranded RNA
into an organism, which degrades the targeted homologous mRNA.
These may include subfractions of small (microRNAs, small nucleolar RNAs) and long RNAs (anti-sense RNA, double-stranded RNA
, and long RNA species) that function as regulators of other mRNAs at the transcriptional and post-transcriptional levels and control protein ubiquitination and degradation, with possible other roles yet to be elucidated.
Scientists believe that this process may have arisen initially to guard against viruses, which sometimes create double-stranded RNA
. Researchers are now exploring the use of RNAi to treat diseases that are caused by inappropriate expression of a human gene or genes or the expression of a viral gene.
The non-RNase H domain of Saccharomyces cerevisiae RNase H1 binds double-stranded RNA
: magnesium modulates the switch between double-stranded RNA
binding and RNase H activity.
The discovery that short, double-stranded RNA
molecules could be generated and processed into siRNA paved the way for the use of these molecules for silencing gene expression in mammals (Elbashir et al.
RNA interference uses double-stranded RNA
, or ribonucleic acid, to block the action of genes in cells.
Mello, Ph.D., in 1998 on the power of double-stranded RNA
as a gene-silencing agent in C.
(1998) after experimental injection of double-stranded RNA
(dsRNA) into the nematode Caenorhabditis elegans resulted in specific silencing or "interference" of genes homologous to the dsRNA.