2000), where four degenerate primers
were used for genogroup I detection.
practice, to synthesize degenerate primer
pairs with each of the two
The cloning strategy for isolating PAL promoter gene fragment from orchid (Dendrobium crumenatum) relied on the use of degenerate primer
corresponding to the conserved region of PAL protein.
An internal piece of CA sequence was obtained from 5-day-old aposymbiotic larvae, using the degenerate primers
dCA-F and dCA-R (Figs.
1999) sequences were aligned and degenerate primer
sets were designed for conserved motifs in the NBS regions of these genes (Fig.
3' RACE reactions employing a degenerate primer
targeted to the conserved FMPERW motif of mitochondrial P450s produced two partial cDNA sequences that were designated DU 3 and DU 13, from the fruit fly species Drosophila arizonae.
Acquiring MSI1 gene's core fragment: According to the homology comparison results among other species' MSI1 gene, we designed 3 degenerate primers
using the primer 5.
Degenerate Oligonucleotide-primed PCR: General Amplification of target DNA by a single degenerate primer
The degenerate primers
designed for this purpose from the conserved region of b-tubulin genes of other fungi was successfully amplified the target gene
The species-specific markers require only conventional PCR (with degenerate primer
sequences) which is readily available, rapid and inexpensive for molecular identification of species in question.
The degenerate primer
set we used here was originally developed by Ito et al.
Evaluation of a degenerate primer
for the PCR detection of human caliciviruses.
To amplify the major portion of Spbtry1, the following degenerate primers
were designed according to the amino acid residues flanking the His and Ser of the serine proteases SP-F and SP-R active site (Table 1) (Waniek et al.
However, immunocapture of ToLCNDV could only be achieved by using the TYLCV-CP antisera followed by PCR detection by using specific and degenerate primers
Due to small, circular single stranded nature of Geminiviruses, different viruses can be easily amplified by PCR by using degenerate primers
(Rojas et al.