In the 5% [O.sub.2] group, there was a higher abundance of ATF4, CDX2, DDIT3, KEAP1, HSF1, OTX2, PAF1, POU5F1 (OCT4), REST, SREBF1, and XBP1 factors that are related to several cell cycle processes.
Most of the transcription factors that presented differences between the studied groups (ATF4, CDX2, DDIT3, HSF1, OTX2, POU5F1, XBP1, HAND1, NANOG, NFKB2, SOX2, and SOD2) also have important functions in RNA metabolic processes, regulating the frequency, rate, and extent of chemical reactions involving RNA [33, 35].
REST, PAF1, DDIT3, and ATF4 were upregulated in the 5% [O.sub.2] group, while HP1 and SOX2 were downregulated in the same group.
Abbreviations: ALL, acute lymphoblastic leukemia; ATF4, activating transcription factor-4; ATF6, activating transcription factor-6; CHOP, C/EBP homologous protein; CPT, cryptotanshinone; DDIT3
, DNA damage-inducible transcript 3; elF, eukaryotic initiation factor; ER, endoplasmic reticulum; ERAD, ER-associated degradation pathway; IPA, Ingenuity Pathway Analysis; IRE1, inositol-requiring enzyme-1; mTOR, mammalian target of rapamycin; PERK, protein kinase RNA-like endoplasmic reticulum kinase; PI3K, phosphoinositide-3-kinase; ROS, reactive oxygen species; UPR, unfolded protein responses; XBPI, X-box protein 1.
Ho et al., "Isochaihulactone-induced DDIT3
causes ER stress-PERK independent apoptosis in glioblastoma multiforme cells," Oncotarget , vol.
Kabjorn-Gustafsson et al., "The myxoid/round cell liposarcoma fusion oncogene FUS-DDIT3 and the normal DDIT3
induce a liposarcoma phenotype in transfected human fibrosarcoma cells," American Journal of Pathology, vol.
We further evaluated the role of DDIT3 expression by studying lipoblast formation in cultured liposarcoma cells treated with adipogenic factors.
Immunohistochemistry (IHC) was performed as described previously  using the DDIT3 specific antibody Gadd153 R20 from Santa Cruz Biotechnology at a dilution of 1:200.
(11) The second most commonly used FISH test was for DDIT3 gene region (12q13), which is split apart in t(12;16) and t(12;22) seen in myxoid liposarcoma.
Regarding the latter example, probes for DDIT3 can detect both the t(12;16) and the t(12;22) translocations in myxoid/round cell liposarcoma.