Caption: Figure 2: Serpine1 gene (PAI-1) expression is higher in irradiated veins (XRT) vs unirradiated veins (0XRT; ddCt
), in relation to GAPDH (da), p <0.001.
Relative levels of mRNA were calculated by the ddCt
method for FKBP51 (2a) or FKBP52 (2b) and represented graphically as fold change relative to HL mRNA levels.
: The ddCt
Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR)," R package version 1.32.0, 2015, https:// www.bioconductor.org/packages/release/bioc/html/ddCt.html.
For petrol people, there's an entry-level 110bhp 1.6 eTorq unit or, if you want petrol but need extra power, there's a 1.4-litre TMair powerplant with either 140 or 170bhp and the option of a DDCT
We analyzed all samples in triplicate and made relative comparison to adult testis control cDNA using the ddCt
According to the characterization analysis, we propose an efficient depth map coding scheme using directional discrete cosine transforms (DDCT
)  adapted to these locally anisotropic features, which can well preserve the boundary accuracy of depth maps and consequently increase the quality of the synthesized views.
As the absolute copy number determination revealed that the expression level of TPM1[kappa] is significantly lower in heart and skeletal muscle, we analyzed our results by 2[conjunction]-(ddCT
) method using 18S rRNA as the reference gene to determine the relative expression of TPM1[alpha] and TPM1[kappa].
By combining the conventional ddCt
method and an original mathematical model, we demonstrated that the difference between [Ct.sub.r1] and [Ct.sub.r2] (i.e., d[Ct.sub.r2r1] = [Ct.sub.r2] - [Ct.sub.r1]) in a given sample can be used as an index that represents the modification rate (see Fig.
Finally, there is a six-speed dual dry clutch transmission (DDCT
), developed by Fiat.
The Fiat Viaggio will be the only car in its segment to exclusively offer sophisticated turbo-charged engines, the 1.4L Tjet in both120 hp and 150 hp outputs matched to either a 5-speed manual transmission or the latest Dual Dry Clutch Transmission (DDCT
) developed by Fiat Powertrain.
leprae recombinant proteins and control (medium alone) using the DDCt
method, (13) where GAP3DH served as a normaliser.
For each sample, the delta delta cycle of threshold (ddCt
) (crossing point) values were calculated as the Ct of the target gene minus the Ct of the GAPDH gene, assuming PCR efficiency equals to 1.
method using Gapdh as a reference was utilized to compute the relative expression of Bcl-2 or Bax.