Assessment of sperm quality through fluorometry and sperm chromatin structure
assay in relation to field fertility of frozen-thawed semen from Swedish AI bulls.
Effects of formaldehyde (FA) exposure on chromatin structure
In addition to histone modifications, histone variants and chromatin remodeling complexes also contribute to altering chromatin structure
In most cells, more than 80% of all genes are "silenced" due to the surrounding chromatin structure
Highly significant differences were found for binucleated cells (Image 2), abnormalities of chromatin structures
, chromatin bridges between the erythroblasts, incompletely divided polypoid cells and karyorrhexis in erythroblasts (Image 3).
The following analyses were carried out for each ejaculate: sperm motility assessment, sperm pH measurement, sperm morphology assessment, sperm chromatin structure
evaluation and cell membrane integrity assessment.
Recent research suggests that ncRNA may have layers of information or signals that control chromatin structure
and epigenetic memory.
We found that stem cells have a different chromatin structure
than somatic (specialized) cells.
Fazzio has uncovered previously unknown processes governing how the chromatin structure
of a cell's DNA influences gene expression in stem cells, conferring on these cells the unique ability to replicate and differentiate into many different types of cells.
The former include aniline/toluidine blue staining and protamine examination by chromomycine A3, while the latter mainly regard the TUNEL (Terminal deoxynucleotidyl tranferase mediated dUTP nick end labelling), COMET (single-cell gel electrophoresis), SCD (Sperm Chromatin Dispersion), SCSA (Sperm Chromatin Structure
Assay), DNA ladder and DNA-break detection FISH (Fluorescence in situ hybridization) assays.
Evenson will also explain his Sperm Chromatin Structure
Assay, how it works and how it can help couples and their doctors determine next steps.
In recent years, it has been demonstrated that the dynamic association of histones and their variants to the genome plays a very important role in chromatin structure
, while their post-translational modifications--acetylation, phosphorylation, methylation, ubiquitylation and ADP-ribosylation--participate in chromatin function and gene regulation (Berger, 2001).
Histonc hyper-acetylation induces a loose chromatin structure
and facilitates topoisomerase-induced DNA strand breaks.
Most genes are tightly packaged by a chromatin structure
- chromatin being what compacts DNA so that it fits inside the nucleus of a cell.
the histones) within the chromatin structure
of the nucleus (Hemberger et al.