Parenchyma showed two types of epithelial cells in adults, chief cells
and oxyphil cells (Figure 1).
(a), (d), (g), (j), and (m) epithelial cells; (b), (e), (h), (k), and (n) parietal cells; (c), (f), (i), (l), and (o) chief cells
. For statistical analysis, a nonparametric Kruskal-Wallis test was used.
Photomicrograph showing chief cells
with pale eosinophilic cytoplasm and round to oval nuclei surrounded by sustentacular cells (hematoxylin-eosin, original magnification X20).
The chief cells
mainly distributed at the body and the bottom of gland, with low columnar or cubic in shape, which located between the parietal cells.
Present study revealed that oxyphil cells were less numerous than chief cells
, placed either at periphery of gland adjacent to capsule or in the centre of gland.
Mills, graduate student Greg Sibbel and Hans Clevers, a geneticist at Utrecht Medical Center, identified markers that show a small number of chief cells
become stem cells even in the absence of serious injury.
C, Multiphoton microscopy reveals individual gastric oxyntic glands that are separated by a mesh of collagen fibers at the deeper part of the mucosal layer ([greater than or equal to] 60 m depth) D, The corresponding light-microscopic image shows there are chief cells
and parietal cells in each gastric gland (hematoxylin-eosin, original magnifications x400 [B and D]).
Paragangliomas are composed of two cell types: chief cells
and sustentacular cells.
(5) The adult parathyroid is composed predominantly of chief cells
, as well as oxyphilic cells, which are mitochondria rich, and transitional oxyphilic cells, which appear to represent an intermediate phase from chief cell
to oxyphilic cell.
Histologically, these tumors appear highly vascular and are composed of clusters (Zellballen, which is German for "cell balls") of chief cells
supported by sustentacular cells and small blood vessels.
(26,28) These are composed of chief cells
and sustentacular cells in a nesting or zellballen pattern.
Histopathologic examination revealed that parts of the tumor contained a lobulated architecture composed of sheets, cords, and follicles of chief cells
, oxyphil cells, and clear cells; these findings were consistent with an adenoma.
Chromogranins are proteins within the chief cells
, and S-100 is produced by sustentacular cells (SY38 is another, less specific, marker for neuroendocrine tumors).
MUC6 is expressed in the cytoplasm of antral pyloric glands but also in mucous neck cells and chief cells
of the gastric body.
Histologically, the cells resemble chief cells
and are uniform, with clear to amphophilic cytoplasm surrounding small, hyperchromatic nuclei (figure, B).