lysis

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Words related to lysis

recuperation in which the symptoms of an acute disease gradually subside

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Cell lysates were generated using RIPA lysis buffer (Dingguo) containing protease inhibitor cocktail (Roche, USA).
Cell lysate solutions (20 [micro]L) were placed in duplicate into the wells of the streptavidin-coated microplate, to which 80 [micro]L of the immunoreagent containing a mixture of anti-histone-biotin and anti-DNA-POD was added.
UVA-induced CatL production and activity were studied with quantitative real-time reverse transcription polymerase chain reaction (RT-PCR), Western blotting, and fluorimetric assay in cell lysates collected on three consecutive days after irradiation.
After incubation for 30 min on ice, the cell lysate was cleared by centrifugation at 13,000 g for 5 min.
coli LPS (1 [micro]g/mL) for 6 h, and the induction of the HO-1 protein was determined by an immunoblot analysis of the cell lysates using a specific antibody against HO-1.
Supernatant was obtained by transfer culture supernatant to a new collecting tube directly; washed the adherent cells three times with Hank's Balanced Salt solution(GibcoUSA)quickly blowed well after adding 1ml Triton X-100(1%) into each well centrifuged 15 minutes at 2000rpm the supernatant was the sample of cell lysate.
The enzyme can be added to the cell lysate to rapidly reduce the large amounts of host-DNA and decrease viscosity, thereby rendering the subsequent unit operations like column chromatography and membrane filtration much more economical, according to the company.
The enzyme can be added to the cell lysate to rapidly reduce the large amounts of host DNA and decrease viscosity, thereby rendering the subsequent unit operations like column chromatography and membrane filtration much more economical.
Nearly 9,000 of products have been added in this launch such as Recombinant Human ZNF80 293 Cell Lysate, Recombinant Human VAMP8 293 Cell Lysate, Recombinant Human YIF1B 293 Cell Lysate, and Recombinant Human BBS5 293 Cell Lysate etc.
No kinetics constants have, to our knowledge, been previously published for a CN activity assay by use of a Jurkat cell lysate.
In cell culture studies, apigenin treatment resulted in cell growth inhibition and induction of apoptosis, which correlated with increased accumulation of IGFBP-3 in culture medium and cell lysate.
In the in vitro assay, SK-N-SH cell lysate was incubated with TCDD ([10.