In the recent reclassification of breast cancers on the basis of copy number expression analysis, the 5q loss is associated with a strong basal-specific signature, including aurora kinase B (AURKB), B-cell CLL/lymphoma 2 (BCL2), BUB1 mitotic checkpoint serine/threonine kinase (BUB1), cell division cycle
associated 3 (CDCA3), cell division cycle
associated 4 (CDCA4), cell division cycle
20 (CDC20), cell division cycle
45 (CDC45), checkpoint kinase 1 (CHEK1), forkhead box M1 (FOXM1), histone deacetylase 2 (HDAC2), insulin-like growth factor 1 receptor (IGF1R), kinesin family member 2C (KIF2C), kinesin family member C1 (KIFC1), methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), TTK protein kinase (TTK), and ubiquitin-conjugating enzyme E2C (UBE2C).
Rabbit monoclonal antibody against activated caspase-3, PARP (cleaved p85), cyclin B1, cyclin-dependent kinase 1 (Cdk1), phospho-Cdkl (Tyr15), cell division cycle
(Cdc) 25c, and GAPDH were obtained from Santa Cruz (Santa Cruz, CA, USA).