A thorough literature survey has revealed that HPLC method for ceftriaxone sodium with combination of other drugs and individually in dosage forms [2-15], microbial bioassay methods [16,17], and spectrophotometric methods in dosage forms [18-22] have been reported for analysis of ceftriaxone sodium.
So, the principle objective of this study was, therefore, to develop a simple, selective, precise, less time consuming, and economical method with a wide linear range and good sensitivity for assay of ceftriaxone sodium in the powder for injection dosage forms.
Therefore, the present study was extended to establish the inherent stability of ceftriaxone sodium under different stress conditions such as, alkaline, acidic, oxidative, and photolytic conditions.
Ceftriaxone sodium was obtained as a gift sample from Orchid Chemicals and Pharmaceutical Ltd.
A stock solution was prepared by dissolving 100 mg of ceftriaxone sodium pure drug in 100 mL of distilled water to get standard stock solution (1mg/mL).
To a set of 10 mL volumetric flasks, aliquot volumes containing the drug were quantitatively transferred from standard stock solution and made up to the mark with distilled water to obtain final concentrations of 5-50 [micro]g/mL of ceftriaxone sodium.
Three levels of standard drug (10%, 20%, and 30%) were spiked individually with the 100 mg equivalent of powder for injection dosage form ceftriaxone sodium and analysed in six replicates during the same day (intraday precision) and six consecutive days (interday precision).
A linear correlation was found between absorbance at [lambda] max and various concentrations of ceftriaxone sodium.
A 2 mL aliquot of standard stock solution of ceftriaxone sodium (1 mg/mL) was taken in four replicates in a volumetric flask (100 mL) and mixed with 10 mL of 0.
For the estimation ceftriaxone sodium from injection (by procuring three brands from market), a portion of powder equivalent to 100 [micro]g of the drug from each brand was accurately weighed and transferred into 100 mL volumetric flask.
Aqueous solution of ceftriaxone sodium showed absorbance maximum ([lambda] max) at 241 nm (Figure 2), and at this wavelength distilled water did not show any significant absorbance.