Briefly, CaCo2 and CEM/ADR 5000 cells were seeded in 96 well-plates and incubated with serial dilutions of doxorubicin as well as each lignans of different concentrations as I mentioned above.
Briefly, CaCo2 and CEM/ADR 5000 cells were seeded in 96 well-plates and incubated with serial dilutions of doxorubicin and each lignan with different concentrations plus digitonin in a non-toxic concentration ([IC.
The cytotoxicity of the six isolated lignans determined against the multidrug-resistant cell lines CEM/ADR 5000 and CaCo2 cells using the MTT assay.
Expression profiles of four different cell lines (COLO, HT-29, CaCo2
and DLD) were compared with the gene markers after treatment with a drug candidate.
Vero and CaCo2
cells, as well as primary cell cultures from Carollia bat lung and kidney, were used.
Concentrations of 5 [micro]M digitonin reduced growth of Caco2
and CEM/ADR5000 cells by 20% (Table I).
corymbosa on human colon carcinoma CaCo2 cells and hepatoma HepG2 cells was appraised.
Human colon carcinoma CaCo2 cells and hepatoma HepG2 cells were maintained in DMEM medium and RPMI 1640 medium, respectively, supplemented with 1% antibiotic-antimycotic and 10% fetal bovine serum.
3] cells/100[micro]l/well for CaCo2 cells and 5x [10.