intestinal epithelial cells (ATCC HTB-37) were maintained in DMEM supplemented with 20% (v/v) heat-inactivated fetal calf serum (hiFCS), 100U/mL penicillin, 100 [micro]g/mL streptomycin, and 2mM L-glutamine at 37[degrees]C in a humidified, 5% C[O.sub.2] atmosphere.
Hierarchical clustering demonstrated that CTC-MCC-41 expression profiles were markedly different from those of HT-29 samples and other colon cancer cell lines (CaCo2
, HCT116 and RKO, C0L0205, and SW620).
cell lines were fixed with paraformaldehyde 4 % sucrose (Sigma-Aldrich) for 20 minutes at room temperature.
Augustijns, "Effect of simulated intestinal fluid on drug permeability estimation across Caco2
monolayers," International Journal of Pharmaceutics, vol.
Finally, miR-145, which is downregulated in colorectal cancer cells (Caco2
, Sw480, HCT116, and HT29) and colorectal tumor tissues, is induced by the PPAR[gamma] agonist (i.e., rosiglitazone) via direct binding of PPAR[gamma] to PPRE in the promoter encoding pre-miR-145 [96,131].
In addition, Caco2
cells express considerably lower amounts of brush border enzymes in comparison to normal enterocytes .
Fink, "Ethyl pyruvate ameliorates intestinal epithelial barrier dysfunction in endotoxemic mice and immunostimulated caco2
enterocytic monolayers," Journal of Pharmacology and Experimental Therapeutics, vol.
Jalpa) or of purified isorhamnetin glycosides on two models of human colon cancer cell lines, namely, HT-29 and Caco2
, representing apoptosis-resistant and apoptosis-susceptible cell lines, respectively, while normal fibroblasts (NIH 3T3) were used as controls.
A former research reported that LPS significantly induced the downregulation and redistribution ofTJ proteins in Caco2
monolayers, as well as promoting the increase of intestinal epithelial permeability .
In this line, procyanidin B2 and procyanidin CI, present in cacao, have been shown to influence the regulation of Apo A-1 in HepG2 and Caco2
cells by increasing their mRNA expression and consequently, Apo A-1 protein levels.
In-vitro model for IBD could be developed by using colon cancer cell line like HCT-116, CaCo2
, SW-620 and others which show some inflammatory responses.
Similarly another study which assessed the possible effects of five nonnutritive sweeteners on cell proliferation morphology and cells' DNA by utilizing Caco2
HT-29 (colon) and HEK-293 (renal) cell lines.
Kim, "Role of hepatocyte nuclear factor 1[alpha] and 1[beta] in the transcriptional regulation of human dipeptidyl peptidase IV during differentiation of Caco2
cells," Biochemical and Biophysical Research Communications, vol.
Colorectal cell lines HT29/219, LS180, SW48, SW480, SW742, SW1116, HCT116, and Caco2
(National Cell Bank of Iran Pasture Institute, Iran) were cultured in a humid incubator at 37[degrees]C, under an atmosphere of 5% CO2 and in 10% fetal calf serum (Cinagen, Iran) containing media (Biosera, UK) of either RPMI 1640 (HT29/219, SW480, SW742, SW1116 and HCT116) or DMEM (LS180, SW48 and Caco2