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The module-related DEGs in the PI3K/Akt (namely, LPAR1, COL5A1, and COL6A2) and TNF signaling pathway (termly CXCL1, CXCL2, and CXCL3) were selected to investigate the effect of melittin on them in BC cells.
MCP-1 and Cxcl3 are chemokines that promote the adhesion of monocytes and induce low-inflammation, and SYE decreased the expression of MCP-1 and Cxcl3.
Meanwhile, the single nucleotide polymorphisms (SNPs) in CXCL3, NME7 and C5 genes are confirmed and gene[PM.sub.2.5] interaction is analyzed to assess its effects on pulmonary function.
The upregulated genes comprise IL-8, IL-1B, CXCL2, CXCL1, CXCL3, interleukin 1, alpha (IL-1A), tumor necrosis factor (TNF), and oxidized low-density lipoprotein (lectin-like) receptor 1 (OLR1/LOX1).
Total liver RNA was extracted from WT or IL-33 KO mice and control (PBS) or L2- MHV3-infected mice (48 h and 72 h PI) and tested by RTqPCR for (a) CXCR1, (b) CXCR2, (c) CXCL1, (d) CXCL2, (e) CXCL3, (f) CXCL5, (g) CCL2, and (h) CCL6.
In the present work, four hub genes were selected including CXCL3, ELF5, PHLPP2, and TIMP1.
Here we analyzed the expression of TCDD-inducible cytokines including CXCL chemokines CXCL1, CXCL2, CXCL3, CXCL5, CXCL7, CXCL10, and CXCL14.
Only 1 marker, COL2A1, was also on our list; however, 2 other markers, CXCL3 and C3, belonged to the same protein family as entries on our list.
C-X-C motif chemokine ligand 2 (CXCL2), C-X-C motif chemokine ligand 3 (CXCL3), C-X-C motif chemokine ligand 8 (CXCL8), and C-X-C motif chemokine ligand 12 (CXCL12) took part in three pathways which were NFkappa B signaling pathway, chemokine signaling pathway, and legionellosis signaling pathway (nodes in three colors).
The HIV-upregulated cytokines/chemokines in the RTECs include inflammatory cytokines CCL20, IL-6-, and the IL-8 related chemokines: CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, and CXCL8 (IL-8).
In addition, the chemokine MIP1a (CXCL3), MIP-1[beta] (CXCL4), RANTES (CXCL5), and Eotaxin (CXCL11) also had higher levels.
Cluster 1 genes (ZBTB1, PML, ZNF44, SIX1, BCL6, ZNF450) were down-regulated by V and involved in gene transcription, whereas cluster 2 genes (IL8, IL1A, PTGS2, DTR, TNFAIP3, CXCL3) were up-regulated and linked to inflammatory response and cell proliferation.
Because the expression of nine cytokines genes, namely TNF, IL6, IL1A, CCL4, CCL22, CCL24, CXCL2, CXCL3, and TSLP, were modulated by USP2 deficiency in HL-60 cells (Figure 2), and overexpressed in HL-60 cells (Figure 3) and isolated mouse macrophages (Figure 4), we next surveyed the Oct-1 and Oct-2 binding sites in the proximal regions of the above cytokines and the CXCL8 gene using public transcription factor binding site databases.
However, contrary to the results from blood analysis, they described no changes in CCL5 and CXCL3 levels in CSF samples from AD patients in comparison to controls.