Therefore, inhibition of cholesterol biosynthesis in cultured cells infected with C trachomatis
might be a key mechanism of attenuation of chlamydial replication in host cells.
However, in the present study, there was no statistically significant difference in the levels of IL-18 between C trachomatis
infected and uninfected males.
(3,6,7) In one reported case, a homosexual man with a history of orogenital contact presented with prominent cervical lymphadenopathy suggesting lymphoma; subsequent serologic studies demonstrated the presence of C trachomatis, and the patient responded to tetracycline therapy.
Although these cultures are specific for C trachomatis infection, the sensitivity of this procedure for oropharyngeal chlamydial infection is unknown and may not fully reflect the true incidence of transmission.
The Becton Dickinson BDProbTec ET system is a new semiautomated system using strand displacement amplification technology for the simultaneous amplification and detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Being an amplification assay, it has the potential to increase the sensitivity and specificity of detection, especially in the case of C trachomatis. The automated system has been designed for higher throughput testing, reduced "hands-on" time, and more rapid time to results.
The BDProbeTec ET C trachomatis and N gonorrhoeae amplified DNA assay was used with the BDProbTec ET system (BD Biosciences, Sparks, Md).
study was to determine the frequency of C trachomatis
infection within sexual partnerships by using highly sensitive polymerase chain reaction (PCR) amplification to detect infection.
We completed a retrospective chart analysis of 347 obstetric patients in a rural family practice residency training program to determine the prevalence, associated risk factors, and screening criteria for cervical C trachomatis
Multiple swabs increase the positivity rate of detection. Culture toxicity due to contamination decreases as the number of specimen collections increases. Elevated serum antibody titers to C trachomatis
, recent patient treatment with antibiotics, culture inoculation delay, and improper thermal transport or storage conditions affect culture outcome. Chlamydial cell culture technique has been refined and improved by the use of direct flourescent antibody confirmation, cycloheximide pretreatment of McCoy cells, and the use of shell vials and nontoxic collection materials.
Logistic regression analysis was used to confirm which factors identified by univariate analysis contributed independently to the risk of the culture being positive for C trachomatis
. The probability of infection by age for women with zero, one, or two risk factors was calculated by logistic regression.
The reference standard for diagnosing C trachomatis
is the McCoy cell culture.
Two C trachomatis
enzyme immunoassay (EIA) kits, the TestPack Chlamydia (Abbott Laboratories, Chicago, Ill) and Surecell Chlamydia (Eastman Kodak, Rochester, NY), are commercially available.
[1,2] Many diseases have been associated with C trachomatis
infection including cervicitis, salpingitis, endometritis, and perihepatitis, with subsequent infertility and ectopic pregnancy.
Although the true incidence of genital C trachomatis
is unknown, it is now thought to be the nation's most prevalent sexually transmitted disease.
From 3 to 5 millio Americans are newly infected with Chlamydia trachomatis each year.[23,24] The annual cost, direct and indirect, of treating mothers and babies infected with C trachomatis
in the United States has been estimated to approach $1.5 billion. Between 1 in 10 and 1 in 20 US women will have chlamydial endocervical infection documented during pregnancy, resulting in about 155,000 infants born to infected mothers each year. This microorganism is now generally considered to be the most common sexually transmitted pathogen in western societies,[25,26] and is said to infect 10% to 20% of sexually active adolescent girls. Chlamydial infection may coexist in up to 45% of patients presenting for treatment of gonorrhea.[27,28]