The sequence of the amplicon was aligned using NCBI-BLAST (https://www.ncbi.nlm.nih.gov/BLAST/) which revealed 98% homology with the FXI gene of Bos taurus followed by Bubalus bubalis. The results of this study provided evidence that there were no heterozygous bulls among the studied lot.
For the preparation of the minced water buffalo meat, buffalo meat (Bubalus bubalis) was taken from the shoulder of the adult female (about 3 to 4 years of age) buffalo carcass conditioned at 4[degrees]C [+ or -] 1[degrees]C for 24 h obtained from the local slaughterhouse (Meat and Milk Institution, Erzurum, Turkey).
He meant that the 1.89 million metric tonnes of beef India exported in 2012-2013 were derived largely from herds of the native water buffalo Bubalus bubalis. This beast is beef, according to the United States Department of Agriculture and the global meat industry, but in India it is known as"buff" and doesn't count as forbidden flesh.