Figure 1 and 2 showed a significant difference between two Brassia rex PLBs sizes, size type 1 (0.00-0.30 cm) and size type 2 (0.31-0.61 cm).
Effects of Preculture Duration and Sucrose Concentration on Brassia Rex PLBs Viability from Sucrose Pretreatment:
In Figure 1 and 2, the effect of sucrose preculture concentration on viability of Brassia rex PLBs is presented.
Effects of Duration Exposure to DMSO Treatments at 24[degrees]C and 0[degrees]C on Brassia Rex Plbs Viability prior to cryopreserved (+LN) and non cryopreserved (-LN) PLBs:
For the TTC test, Figure 5 and 6 showed the viability of Brassia rex PLBs after exposure to variety length of DMSO treatment at two different temperature (24[degrees]C and 0[degrees]C), with (+LN) and without (-LN) their subsequent storage in LN.
For the overall of Part II experiments, using TTC test, it can be concluded that size type 2 Brassia rex PLBs which was underwent 10 min DMSO treatment maintain at 0[degrees]C have the best viability (Fig.
Por otra parte, el numero de estratos es variable en las especies de Ada, Brassia
y Miltonia (clado G), Cyrtochilum (clado F), Erycina y Tolumnia (clado I), Oncidium (clado E), Trichocentrum (clado A) y Trichopilia (clado B) (Cuadro 1).