We used Boyden chamber
and wound-healing assays to study the effects of OH-PBDEs on the migration of SKBR3 breast cancer cells, which has been shown to be regulated by GPER pathways (Pandey et al.
The results of the CCK-8 proliferation assay revealed that FMOD-siRNA-treated RPE cells displayed decreased proliferation compared with the control group: (a) statistical analysis of CCK-8 proliferation assay; statistical analysis of the Boyden Chamber
assay revealed that the number of cells that passed through the membrane in the FMOD-siRNA group was significantly lower than the number in the control group: (b) statistical analysis of Boyden Chamber
assay; (c) NS-siRNA-treated RPE cells; and (d) FMOD-siRNA-treated RPE cells.
The influence on cell migration and invasion was further assessed by modified Boyden chamber
. As shown in Figure 3A-D, both migration and invasion were repressed by a single treatment of IL-27 or sorafenib (all P<0.05 compared to control).
We used two modified Boyden chamber
assays to investigate the migration and invasion of ESCC cell lines.
In addition, we used a modified Boyden chamber
assay to evaluate the chemotactic activity of HGF on cell migration .
Chemotaxis Assay in Modified Boyden Chambers
. OE-MSCs, cultured in serum-containing medium, were detached by trypsin/EDTA, counted, and seeded into the upper chamber of cell culture inserts for 24-well plates with translucent polyethylene terephthalate (PET) filter membranes with 8 [micro]m diameter pores (BD Falcon[TM], BD Biosciences, Le Pont de Claix, France) at a density of 1.2 x [10.sup.4] cells per insert, in a final volume of 200 [micro]L of serum-free medium with 1% ITS-X.