Human embryos with unevenly sized blastomeres
have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation.
After chromosome segregation, the plane of the first cleavage consistently occurs parallel to the minor axis and results in two equally sized blastomeres
Perfecting this assisted reproduction technique may provide possibilities to obtain embryos of a determined sex selectively by means of blastomere
Once the blastomere
is collected, it is normally cocultured with the parental biopsy embryo in the medium containing fibronectin and laminin.
that absorbed the PL became the large blastomere
Sequentional comprehensive analysis on polar bodies, blastomeres
and trophoblast: insights into female meiotic errors and chromosomal segregation in the preimplantation window of embryo development.
Antibody fluorescence was present on the nuclei of blastomeres
in embryos from the antibody group, while no significant antibody fluorescence was observed in the embryos from groups of PBS, blank control, and antibody group for control.
Normal cleavage was defined on the basis of data from time-lapse microscopy as cleavage of the zygote to two blastomeres
and subsequent cleavages of each blastomere
to two blastomeres
The increase of cell segmentation enhances the degree of compaction between blastomeres
and marks the end of the ball stage (Wolpert et al., 2000), also referred to as morula and early blastula (Andrade-Talmelli et al., 2001; Reynalte-Tataje et al., 2001; Romagosa et al., 2001; Anjos and Anjos, 2006; Ninhaus-Silveira et al., 2006; Faustino et al., 2007; Paes, 2008; Nakaghi et al., 2013).
The MDA products of the other 6 blastomeres
nontransferred were also used for All-in-One target region sequencing.
The Ciona embryo was historically regarded as a mosaic embryo, (26,27) in which different blastomeres
that inherit different localized maternal materials assume different developmental fates.
It reviews current methods and experiences, then provides an atlas of normal and abnormal human preimplantation development; micromanipulation and biopsy of polar bodies, blastomeres
, and blastocytes; nuclear transfer techniques and the prospect for artificial gamete formation; and preimplantation diagnosis for aneuploidies, translocations, and single-gene disorders.
In general, in the day 3 embryo images, there are several important morphological indices for the evaluation of embryo viability: the number of blastomeres
, uniformity of blastomeres
, and symmetry .
Specific topics include germ-cell differentiation from pluripotent cells, adult stem-cell population in the human testis, embryonic stem cells from blastomeres
maintaining embryo viability, in utero hematopoietic cell transplantation, and the metabolic framework of pluripotent stem cells and potential mechanisms of regulation.
Embryos were scored according to the number, size of the blastomeres
and the amount of anucleate fragmentation: type 1, embryos had equal-sized blastomeres
and anucleate fragmentation; type 2, embryos had blastomeres
unequal in size and less than 20% fragmentation; type 3, embryos had 20%-50% fragmentation; and type 4, embryos had greater than 50% fragmentation.