VOCs detected were finally compared to those found in ascomata of T.
DNA extraction, PCR and sequencing: Ascomata, previously brushed under tap water to detach soil debris, were superficially sterilized with alcohol (90 %), deprived of peridium, cut in thin slices and frozen at - 20 oC until molecular analyses were performed.
Morphology:Macroscopic and microscopic features of ascomata of the three Tuber species investigated,except for spherical spores percentage found in T.
bellonae although more ascomata (if possible considering the already mentioned rarity of this taxon in nature) should be analyzed to support this assumption.
aestivum specimens (subgroup A) while a lower bootstrap support (< 50 %) was observed for the other 4 ascomata of T.
bellonae ascomata from Basilicata was 49,379 u m3 instead of 15,072 +/-2.
bellonae ascomata was not accomplished in the past.