The latter can occur as a result of incomplete fractionation of the CDT and non-CDT isoforms by anion-exchange
These results indicate that anion-exchange
FPLC is a powerful method for evaluating the variability in protein composition of peas.
We used a polystyrene divinylbenzene copolymer resin linked to a quaternary amine anion-exchange
moiety to remove, from ether extract, nonacidic species and those showing weak adsorption to polystyrene.
The mobile phases for anion-exchange
chromatography (PRP-X100) were aqueous solutions of N[H.sub.4]HC[O.sub.3] (20 mmol/L) at pH 8.0, or adjusted to pH 9.0, 9.5,10.0, or 10.7 with aqueous ammonium hydroxide.
We used a 50 X 5 mm MonoQ HR 5/5 anion-exchange
column (Pharmacia Biotech).
LC-MS/MS results were compared with a modified Watson-Schwartz screening method and with quantitative anion-exchange
This can be achieved by chromatographic (e.g., anion-exchange
) or electrophoretic (e.g., IEF) methods, using the different charges and pIs of CDT and non-CDT isoforms.
microcolumn separation of CDT- and non-CDT-transferrins.
SDS-PAGE and immunoblotting of the peak fractions (I, II, and III) obtained by anion-exchange
chromatography showed that the PSA in peak I contained a single 30-kDa band reacting only with the antibody to PSA (Fig.
We have had in use in our laboratory a GC/MS method for the measurement of methylmalonic acid (1VIMA) in plasma, based on solid phase anion-exchange
extraction, derivatization with cyclohexanol, and separation with quantification using GC/MS (2).
Commercially available sets of reagents for determination of CDT use in vitro iron saturation of transferrin to eliminate [Fe.sub.1]- and [Fe.sub.0]-transferrins and anion-exchange
microcolumns to fractionate (separate) the non-CDT- and the CDT-isotransferrins.
A fast-protein liquid chromatography (FPLC) system (Pharmacia) with three types of columns are used for the separation and identification of As-protein bindings: size-exclusion (SEC) (Superose HR 10/30 analytical column and Sephadex G-50 XK 50/30 preparative column), anion-exchange
(MonoQ HR 16/10), and affinity (AFC) [N-hydroxysuccinimide (NHS)-activated HiTrap Superose[R] HR 10/2, coupled with goat anti-human transferrin].
In particular, solvent extraction and anion-exchange
methods before gas chromatography (GO) or gas chromatography-mass spectrometry (GC-MS) analyses have been more widely used for detection in biological samples.
chromatography was performed with a Mono-Q-HR 5/5 column (Kabi Pharmacia Diagnostics, Uppsala, Sweden) connected to a prefilter for HR 5/5.
DE-53 cellulose anion-exchange
resin was obtained from Whatman (Fairfield, NJ; cat.