Key words Anion-exchange
chromatography with pulsed amperometric detection using a gold working electrode can be used to determine various carbohydrates in different foodstuff matrices.
In a third step, they were further separated by anion-exchange
chromatography at pH 8.
Varcoe studied the effect of humidity on the ionic conductivity of alkaline anion-exchange
membranes and found that at high humidity levels, the membranes were good conductors.
Baseline separation was achieved for all six species as well as for the internal standard (potassium hexahydroxy antimonate V) in a single chromatographic run of less than 30 min, using an ammonium carbonate buffer gradient (between 10 and 50 mM) at ambient temperature, in conjunction with cation- and anion-exchange
columns in series.
This new column family consists of one anion-exchange
-quaternary ammonium - column chemistry and two cation-exchange column chemistries - a carboxy methyl and a sulfopropyl - each contained on monodisperse, non-porous particles.
According to the results of XRD, it could be concluded that a great deal of SMAS intercalated into the stacked HT galleries during the anion-exchange
Until now, the methods have required separate isoformseparation and transferrin-quantification steps, such as isoelectric focusing and immunofixation, or anion-exchange
column chromatography and immunoassay.
Resin-exchangeable P was determined using anion-exchange
resin strips (McLaughlin et al.
The procedure included iron saturation followed by CDT extraction onto anion-exchange
disposable cartridges and a TIA.
As a follow-up to that initial work, a multidisciplinary team of scientists from DuPont, the Massachusetts Institute for Technology (MIT) and the University of Illinois worked together to discover a new method for separating carbon nanotubes using single stranded DNA and anion-exchange
HPLC separation of transferrin glycoforms was performed on a SOURCE[R] 15Q anion-exchange
column (Amersham Biosciences) by linear salt gradient elution, and quantification relied on measurement of the absorbance of the iron-transferrin complex at 470 nm (17).
Peptides derived from FXIIIA that were identified by DPD were not observed by SELDI-MS on chips with cation-exchange (WCX chip), anion-exchange
(SAX chip), or metal-chelating (IMAC chip) surfaces.
Several types of analysis are available to quantify NANA, including colorimetric methods (10,11), thin-layer chromatography (12), gas chromatography-mass spectrometry (12,13), HPLC with fluorescence detection (14), HPLC with ultraviolet detection (15), enzymatic assays (14,16), high-performance anion-exchange
chromatography with pulsed amperometric detection (HPAE-PAD) (17,18), and HPLC with mass spectrometry (HPLC-MS) (19,20).
5 to 200 kDa, we used an automated high-throughput, multidimensional strategy involving anion-exchange
fractionation, two different ProteinChip surfaces, and the SELDI ProteinChip Biomarker System.