Isolation and molecular detection of Clostridium chauvoei
alpha toxin gene from clinical cases of black quarter in cattle.
perfringens and the detection of the
alpha toxin gene by polymerase chain reaction, but without any traces of the beta toxin, enterotoxin, epsilon toxin, or iota toxin.
Staphylococcus aureus in vitro secretion of
alpha toxin (hla) correlates with the affiliation to clonal complexes.
Alpha toxin is composed of two domains which are associated with phospholipase C activity (N-domain, 1-250 residues) and membrane recognition (C-domain, 251-370 residues), respectively.
perfringens
alpha toxin (lecithinase) on lecithin of egg yolk onto enriched egg yolk agar medium which appeared as pearly opalescence zone surround the colonies while this reaction was inhibited by C.
The
alpha toxin is reported as hemolytic, necrotic and histotoxic (Quinn et al., 2005).
Effects of subinhibitory concentrations of antibiotics on
alpha toxin gene expression of methicillin sensitive and methicillin resistant Staphylococcus aureus isolates.
perfringens lethal toxins i.e., the
alpha toxin gene (cpa), the beta-toxin gene (cpb) and the CPE gene (cpe).
Polymerase chain reaction assay was employed to detect the virulence toxin genes like
alpha toxin gene (cpa), beta toxin gene (cpb), epsilon toxin gene (etx), iota toxin gene (iA), enterotoxin gene (cpe) and beta2 toxin gene (cpb2) of C.
Several years ago, it was believed that
alpha toxin was the main virulence factor involved in these cases, although some recent studies have shown that this is not completely true (MCCOURT et al., 2005; BUESCHEL et al., 2003).
The
alpha toxin is one of the major toxins involved in the pathogenicity of the disease (Useh et al.