Genes: luxA, alkanal
monooxygenase subunit alpha; luxB, alkanal
monooxygenase subunit beta; luxC, fatty acid reductase; luxD, acyl transferase; luxE, acyl-protein synthetase (long-chain-fatty-acid-luciferin-component ligase); cdd, cytidine/deoxycytidine deaminase; pyrE, orotate phosphoribosyltransferase; DCK, deoxycytidine kinase.
The most relevant PL oxidation products can be sorted into (i) full chain length products (hydroperoxides, hydroxides, and epoxides), (ii) full-chain rearranged products (isoprostanes and isofurans), and (iii) truncated products (alkanals
, alkenals, and hydroxyalkenals) .
Lipid peroxidation is an auto-catalytic free radical mediated destructive process, whereby polyunsaturated fatty acids in biomembranes undergo degradation to form lipid hydroperoxides, which latter decompose to form a wide variety of products including ketones, fatty acids, low molecular weight hydrocarbons, alkenols and alkanals
, in particular malonaldehyde (MDA) (Zeyuan et al.
Preanalytical factors aside [8-10], interfering chromogens include several other ordinary side-products of lipid autooxidation, alkanals, alkenals, and alkadienals as well as bile pigments, cyclic peroxides, carbohydrates, and amino acids X11-18].
Various alkanals such as formaldehyde, acetaldehyde, propanal, and hexanal, which have been shown to both reduce MDA recovery and likewise coelute with the MDA-TBA adduct [12 and N.R.