Four groups containing seven BALB/c mice were immunized subcutaneously with 10 [micro]g of OVA (InvivoGen, USA) in combination with 15% (v/v) Alhydrogel [2% Al(OH)3, InvivoGen, USA], 0.5% (v/v) xanthan polysaccharide, or 0.9% (v/v) endotoxin-free sterile saline (<0.1 EU/mL, InvivoGen, USA).
In contrast, Alhydrogel exhibited growth inhibition rates above 50%, demonstrating cytotoxic effect in fibroblasts cells.
Observation of the cells treated with Alhydrogel 15% (Figure 2(b), (C)) identified damaged cellular membrane with uptake by PI, with morphological alterations when compared to the untreated control.
In addition, whereas mice that received OVA in Alhydrogel mounted a strong IgG response, mice vaccinated with OVA-xanthan exhibited only IgG1 response.
Two cynomolgus macaques were immunized then twice boosted with a recombinant plague vaccine consisting of rF1 and rV antigens with an alhydrogel
adjuvant (plague (recombinant) vaccine suspension, 873082-H01, Batch 7051, Avecia Biologics Ltd., UK).