Two fucose-specific lectins, Aleuria aurantia
lectin (AAL) and Lens culinaris agglutinin (LCA), bound approximately 4% and 8% of plasma proteins, respectively; Lycopersicon esculentum lectin (LEL), which targets polylactosamine, and HPA (see Clinical Tests Based on Cancer-Related Changes in Glycosylation) selected [less than or equal to] 0.1% of the proteins contained in plasma.
Enzyme-linked immunosorbent assay (ELISA) for Aleuria aurantia
lectin-reactive serum cholinesterase to differentiate liver cirrhosis and chronic hepatitis.
A lectin immunoassay using the fucose-specific Aleuria aurantia lectin (AAL) was adapted for use in the Auto DELFIA (Wallac), with europium-conjugated streptavidin used for signal detection.
Discrimination of liver cirrhosis from chronic hepatitis by measuring the ratio of Aleuria aurantia lectin-reactive serum cholinesterase to immunoreactive protein.
The Aleuria aurantia lectin (AAL) was used in an ELISA for analysis of fucosylation of serum cholinesterase in liver disease (10).
Purification and properties of a novel fucose-specific hemagglutinin of Aleuria aurantia. Biochemistry 1980;19:2841-6.