Optimisation de la transformation genetique de la ponme de terre par Agrobacterium tumefaciens
. Utilisation de la resistance a l'hygromycine comme marqueur selectif.
Transient gene expression in plant cells mediated by Agrobacterium tumefaciens
: Application for the analysis of virulence loci.
strain EHA105, harbouring the binary vector pCAMBIA1301, was used to transform rice calli.
Klimaszewska, "Organic nitrogen composition of the tissue culture medium influences Agrobacterium tumefaciens
growth and the recovery of transformed Pinus radiata embryonal masses after cocultivation," In Vitro Cellular & Developmental Biology Plant, vol.
The transfer of DNA from Agrobacterium tumefaciens
into plants: a feast of fundamental insights.
Susceptibility to Agrobacterium tumefaciens
and cotyledonary node transformation in short-season soybean.
In conclusion, a study of cancer incidence among those exposed to Agrobacterium tumefaciens
in the laboratory and in the field is needed.
Wheat, rice, sorghum and barley are among those monocot plants that can be efficiently transformed by Agrobacterium tumefaciens
(Hiei et al.
Brunca via Agrobacterium tumefaciens
considering several factors like bacterial concentration, incubation of the pre-culture and co-cultivation, antibiotics used and lethal concentrations of selective agents.
Sterilized potato pieces were inoculated with 50 [micro]L of Agrobacterium tumefaciens
Four bacterial strains were selected including Pseudomonas aeruginosa (ATCC 27853), Proteus mirabilis (ATCC 7002), Agrobacterium tumefaciens
(ATCC 5577) and Bacillus subtilis (ATCC 6051).
However, it is not trivial to establish a wheat genetic transformation protocol, notably using Agrobacterium tumefaciens
; because, in addition to the multitude of factors affecting this technique (ZIEMIENOWICZ, 2014), the response window is considered narrower compared to other cereals (HIEI et al., 2014).
The screening for their antitumor activities was performed against Agrobacterium tumefaciens
by following the potato disc tumor assay.
Vector Construction and Agrobacterium tumefaciens