The protocol of Agrobacterium
meditated transformation is relatively simple, straightforward, economical, and the most important; it results in the insertion of single transgene (Hansen and Wright, 1999).
Brunca via Agrobacterium
tumefaciens considering several factors like bacterial concentration, incubation of the pre-culture and co-cultivation, antibiotics used and lethal concentrations of selective agents.
tumifaciens MTCC 609, Staphylococcus aureus MTCC 737 and Escherichia coli MTCC 46 were procured from Microbial Type Culture Collection, Institute of Microbial Technology, Chandigarh, India.
Four bacterial cultures including Bacillus subtilis (one Gram positive), Pseudomonas aeruginosa, Proteus mirabilis, Agrobacterium
tumefaciens(three Gram negative bacteria) were used to check the antibacterial potential of Cu nanoparticles (5% to 25% concentration) synthesized from fenugreek seed extract.
transformation of elite wheat germplasm without selection.
Rhizobium radiobacter was firstly isolated from human clinical specimens by Lautrop in 1967.5 Rhizobium radiobacter (Agrobacterium
radiobacter) is a ubiquitous, aerobic, motile, oxidase positive, non-spore forming soil organism.6 It is usually known as a plant pathogen however can lead infections in human very rarely especially in immunocompromised patients.7 The type of contamination in patients with Rhizobium radiobacter infection remains hypothetical.
Sawada, "A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium
Conn 1942 and Allorhizobium undicola de Lajudie et al.
served as a negative control.
(1998) Crown gall of grape: biology of Agrobacterium
vitis and the development of disease control strategies.
Currently, the transgenic methods commonly used for most of the plants include the Agrobacterium
tumefaciens mediated technique and particle bombardment.
serriola, transformed with Agrobacterium
rhizogenes bearing the rolB gene.
The vector was transformed into Agrobacterium
tumefaciens AGL1 strain by electroporation method for plant transformation.
Several bacterial species belonging to different genera including Pseudomonas, Agrobacterium
, Flavobacterium, Arthrobacter, Enterobacter, Stenotrophomonas, Sphingomonas, Burkholderia, Serratia and Bacillus, have been characterized possessing the capability to hydrolyze OP compounds (Horne et al., 2002; Li et al., 2007; Pakala et al., 2007; Yang et al., 2010; Ali et al., 2012).