own Ti-plasmid, and T-DNA region of the Ti-plasmid is excised, transformed into the host plant tissue and integrated into the genome of the plant (Ali et al.
tumefaciens-mediated transformation of maize embryos using a standard binary vector system bbreakthrough technologies.
1998) Crown gall of grape: biology of Agrobacterium
vitis and the development of disease control strategies.
Multiplex PCR assay for identification of commonly used disarmed Agrobacterium
Enzyme chlorpyrifos hydrolase obtained from the strain was belong to subclass phosphotriesterase that hydrolyzes the phosphotriester bond in chlorpyrifos, the mechanism of action is just like phosphotriesterases isolated from Flavobacterium, Pseudomonas and Agrobacterium
(Mulbry et al.
This protocol can be utilized to generate transgenics using gene gun or Agrobacterium
mediated transformation systems.
Proposal for rejection of Agrobacterium
tumefaciens and revised descriptions for the genus Agrobacterium
and for Agrobacterium
radiobacter and Agrobacterium
Mukeshimana G, Ma Y, Walworth AE, Song G, Kelly JD (2012) Factors influencing regeneration and Agrobacterium
tumefaciens-mediated transformation of common bean (Phaseolus vulgaris L.
We learned how Agrobacterium
manages to put genes into plant cells, and then we copied that process.
This method has been applied successfully for many cultivated crops especially monocots like wheat or maize for which transformation using Agrobacterium
tumefaciens has been less successful.
Transient expression in leaves by infiltration of Agrobacterium
tumefaciens (also known as agroinfiltration) is routinely used in several areas of investigation in molecular biology, not only to express external genes or gene silencing (11) but also to evaluate possible promoter sequences and to detect protein interactions (13).
The Ti plasmid of Agrobacterium
tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-Acyl homoserine lactonase activity.
Transgenesis induced by infection with Agrobacterium
tumefaciens or Agrobacterium
rhizogenes and bombardment of cells with micro-projectiles covered with DNA are the principal techniques with which it has been possible to obtain transgenic plants from different plant species.
Towards that end, she and colleagues enlisted the aid of Agrobacterium
tumefaciens, a soil bacterium commonly used in genetic engineering procedures to endow plants with new traits.
Desde el inicio del uso de la bacteria Agrobacterium
tumefaciens en los anos 80, se revoluciono la obtencion de plantas resistentes a diversos patogenos de una manera rapida y sencilla, siendo considerado hasta la fecha como un proceso importante en la biotecnologia vegetal (Petti et al.