Single Domain Antibodies Affinity Maturation
In this situation, after foreign antigen recognition in germinal centers, B cells undergoing affinity maturation
, which improves the receptor affinity by somatic mutations, accidentally would give rise to auto-reactive cells.
Affinity and avidity studies have shown that continuous or repeated contact with antigen leads to an antibody affinity maturation
In the process of proliferation, effect cells generate a large number of antibodies, and then the antibodies duplicate and mutate to make affinities gradually increase and eventually reach affinity maturation
. The clonal selection theory simulates the process of evolution of immune cells, which can learn and memorize the modes of antigens.
Through the proportional clonal, mutation and clonal selection, the proposed algorithm performs a greedy search which reproduces antibodies and selects their improved offspring after the affinity maturation
GenScript offers extensive antibody drug services that include antigen production, hybridoma development and stabilization, antibody sequencing, chimeric antibody, antibody humanization, affinity maturation
, epitope mapping, antibody and protein characterization, in vitro assay and screening, in vivo efficacy and safety study, antibody transient expression, stable cell line, and scale up production.
The clonal selection algorithm (CSA) is a special kind of immune algorithms using the clonal expansion and the affinity maturation
as the main forces of the evolutionary process.
The ProteOn is also used downstream to monitor the preservation of Fab affinity after the affinity maturation
1A) has proven to be a highly effective platform for various directed evolution applications, including affinity maturation
(10-12) and changes in specificity (13, 14).