The three methods used to measure glycated hemoglobin are immunoassay, high performance liquid chromatography (HPLC) and boronate affinity chromatography
1c] assays can be divided into methods that use molecular charge (CE-HPLC and electrophoresis) and methods that use molecular structure (immunoassays, boronate affinity chromatography
, and mass spectrometry).
In scale up of affinity chromatography
for purification of enzymes and other proteins the following basic assumptions are needed to formulate the rate model: (1) the column is isothermal, (2) the packing particles are considered spherical and possess the same size, (3) the diffusion in the radial direction is negligible, (4) there is no convective flow inside particle macropores, (5) the packing density is even along the column length, and (6) the mass-transfer and kinetic parameters are constant.
The authors compare the different protein purification procedures, walk through the steps in planning a lipase purification strategy by affinity chromatography
, and highlight the importance of assessing purified proteins by polyacrylamide gel electrophoresis.
To overcome the difficulties of previous studies that used indirect methods, we developed a new affinity chromatography
method that can be used in combination with nano-liquid chromatography electrospray ionization tandem mass spectrometry (nano-LC-ESI-MS/MS).
The Hemopurifier converges the established scientific principals of affinity chromatography
and hemodialysis as a means to augment the immune response of clearing viruses and toxins from the blood before cell and organ infection can occur.
and ultracentrigugation copelleting were used to study the binding of rabbit muscle and yeast enzymes to rabbit muscle and yeast actin.
Later, some other related subjects such as alginate and lectin chemistry, applications in affinity chromatography
, immunochemical investigations, etc.
The form-stable silica matrix is precharged with Ni2+ ions and allows purification on the principle of Immobilized Metal Ion Affinity Chromatography
Objective: Molecular imprinted polymers and electrospun nanofibres are both hot topics individually in the biomedical sciences, with applications including tissue engineering, regenerative medicine, drug release, affinity chromatography
Boronate affinity chromatography
measurement of glycated Hb is generally considered resistant to interference by most Hb variants including HbC and S trait (5, 6).
New development in Affinity Chromatography
is the largest segment, in terms of value.
UK) presents a basic biotechnology laboratory guide to various protein purification techniques, including solubility, ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography
, and size exclusion chromatography.
They cover cloning and the common steps in isolation--salting out, centrifugation, electrophoresis, ultrafiltration, chromatography, Western blotting, and affinity chromatography