Glycolysis uses simple carbohydrates, such as glucose, as substrate and produces two carbon dioxide molecules per glucose as well as adenosinetriphosphate (ATP) for energy demands in the cellular level.
Double-stranded DNA oligonucleotides and the PCR product were labeled with [[[gamma]-.sup.32]P]ATP (adenosinetriphosphate, 3,000 Ci/mmol; Amersham Pharmacia Biotech, Roosendal, the Netherlands) using T4polynucleotide kinase (MBI Fermentas, Hanover, MD, USA).