For actin visualization, the slides were incubated with TRITC-Phalloidin (dilution 2 [micro]M, Sigma) in PBS with 1% BSA and 0.2% Triton X-100 for 30 min.
Confocal microscopy images of F-actin stained with Phalloidin showed that, in PEG 35 pretreated livers, filamentous actin associated with the membrane microfilamentous network and the pericanalicular band are kept preserved (Figure 5(a)).
This study describes the isolation, cloning and phylogenetic studies of a partial sequence of actin gene from the fungus Trichoderma harzianum E-58, which was grown in Vogel's medium at 28oC and pH 5.5, with glucose as a carbon source.
Keywords: Actin gene; Trichoderma harzianum; Cloning; E.
One of the intriguing properties of Gc-globulin is its ability to bind extracellular actin released by necrotic cell destruction into the circulation and, thus, to protect against disseminated intravascular coagulation induced by polymerized forms of actin (2).
Other clinical situations such as septic conditions have also been shown to be associated with low Gc-globulin concentrations and actin complexation (16), but the specific role of Gc-globulin has yet to be defined.
Moreover, in the motor cortex of the undamaged hemisphere, we observed an important reexpression of nestin and actin in GH-treated rats, but not in vehicle-treated or control animals, which probably indicates the development of compensatory mechanisms to achieve a functional recovery.