Bioactive metabolites from actinomycetes
were produced by agar surface fermentation.
Conventionally, identification of endophytes was done based on morphological characteristics for bacteria, fungi, and actinomycetes
and with the help of biochemical tests for bacteria and actinomycetes
Therefore, screening, isolation, and characterization of promising strains of actinomycetes
producing potential antibiotics and other therapeutics have been a major part of research [11,12].
The soil sediments were subjected to heat pretreatment at 50[degrees]C for 60 min for isolation of marine actinomycetes
. One gram of each soil samples was then suspended in sterile water and incubated at 28[degrees]C in a rotary shaker at 150 rpm for 1 hour.
farcinica is still though not often reported as a causal agent of bovine farcy when diagnosis based on morphological traits, which fail to discriminate Nocardia from other mycolic acid-containing actinomycetes
, is used [31-40].
Perhaps the most important organisms found in WDB are actinomycetes
. No, not fungi, not mold, not black molds, not toxic black molds, but these filamentous bacteria are only recently becoming recognized in clinical dust samples as major players in adverse human health effects.
by using traditional culturing techniques has been challenging, advent of molecular biology techniques, such as 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, has improved identification of aerobic actinomycetes
RERP were positively correlated with the concentration of [Na.sup.+] (r = 0.86) and silt (r = 0.86) as well as the population of actinomycetes
(r=0.93) and Trichoderma (r = 0.82) in the soils.
The plates were incubated at 30[degrees]C for 5 days for Actinomycetes
, and at 37[degrees]C for 24 hours for Eubacteria 12.
Summary: Chromatographic purification of the culture broth of Actinomycetes
CDS1 provided five new (1-5) and three known (6-8) metabolites.
Among 23,000 bioactive microbial secondary metabolites, 45% (10,000) of them are produced by actinomycetes
from which ~7,600 compounds are produced by only Streptomyces species.
and fungi that could be cultured in rhizosphere soil samples were evaluated using the plate dilution method (Li et al.
According to Garbaye and Frey-Klett , it is well established that some strains of Rhizobium can exhibit nitrogenase activity when grown in vitro on different media, but such activity has not been reported for Actinomycetes
strains isolated from root nodules of N2-fixing nonlegumes .
Consideration may also be given to concomitant bacteria that may be resistant to penicillins and continue the inflammation after the actinomycetes
have been treated; for example, dual therapy may be utilised with metronidazole for anaerobic cover in addition to penicillin .