97 Microbiologic Analysis 96 Culture positive Acid-Fast stain
positive 18 18.
Physicians need to alert the laboratory when they suspect Cyclospora so that a modified acid-fast stain
can be performed.
With the acid-fast stain
, these illnesses would be misidentified as TB and, in most instances, also would be reported as treatment failures.
Morphologic characteristics, together with negative routine acid-fast stains
(Ziehl-Neelsen, auramine-rhodamine), differentiate nocardia from mycobacteria.
Eight patients had no sputum specimens submitted for acid-fast stain
Lesions can be screened for mycobacteria using an acid-fast stain
However, direct and acid-fast stain
microscopic examination of stool specimens from nine of the house staff physicians and one of the other employees revealed the presence of CLB.
A direct acid-fast stain
showed many acid-fast bacilli.
They were stained with Kinyoun's carbol fuchsin modified acid-fast stain
and examined by oil immersion microscopy.
parvum oocysts were processed with acid-fast stain
(AFS) (8) to check for normal cellular morphologic features.
To confirm the presence of acid-fast bacteria, tissue sections were stained using multiple commercially available acid-fast stains
(Fite's Stain Kit, American Master Tech Scientific Laboratory Supplies, Lodi, California; Kinyoun's Stain, Gibson Labs, Lexington, Kentucky; Ziehl-Neelsen Stain, Gibson Labs, Lexington, Kentucky).
Smears performed from the colonies revealed Gram-positive microorganisms; partially acid-fast stains
(modified Kinyoun acid-fast) were also positive (Figure 2) but Kinyoun stains were negative in all isolates.
However, no recent studies have been published that evaluate the sensitivity of modified versus standard acid-fast stains
for diagnosis of cutaneous RGM infections.
Microbiology stainers provide high-quality Gram staining and different natural or fluorescent acid-fast stains
Currently, definitive diagnosis of mycobacteriosis in birds is accomplished by demonstrating organisms in organ biopsy or aspirate samples with acid-fast stains
, culture, or molecular methods.