Comparison of the modified fluorescent method and conventional ZiehlNeelsen method in the detection of
acid fast bacilli in lymph node aspirates.
Only in BB, BL and LL skin smears and biopsies were found to be positive for
acid fast bacilli (Table 4).
As microscopy for
acid fast bacilli was positive but Xpert MTB/RIF was negative, the possibility of NTM infection with lymphadenitis was considered and specimen cultures were extended for NTM.
In total, three
acid fast cultures were sent to the lab for analysis but their results would not come out for some time from the Health Dept.
KEY WORDS: Haematoxylin and eosin;
acid fast bacilli; Kinyoun; Ziehl Neelsen; Lymph node.
Mycobacterium fortuitum: um novo bacilo acido-resistente patogenico para o homen [Mycobacterium fortuitum: new
acid fast bacillus pathogenic for man].
Our previous study4 showed 42.5% sensitivity of
Acid Fast Staining (AFS) staining whereas in another study5 sensitivity was 50%.
Intestinal impression smears of the later (06 animals) were found as positive for the incidence of
acid fast bacilli and later on, their tissue sections were cautiously examined in the laboratory.
These milk samples were smeared for
acid fast staining and also inoculated on Stone brink's media and incubated at 37degC for six to eight weeks.
Decreasing reliability of
acid fast smear technique for detection of tuberculosis.
The cells are
acid fast and do not form cords or branches.
Additional microbiological workup was ordered on wound specimens collected using sterile swabs and anaerobic culturettes for both aerobic and anaerobic cultures, along with
acid fast stains and cultures.
However
acid fast staining of section did not show presence of acid-fast bacilli.
A slit skin smear examination revealed presence of
acid fast bacilli.
Acid fast bacilli should be searched for in skin biopsies and culture, and a polymerase chain reaction should be done as well.