1.1.1.35): 50 mM imidazole, pH 8.0, 0.2 mM NADH, 1 mM KCN, 0.1 mM acetoacetyl CoA (omitted for control);
2.8.3.2): 50 mM imidazole, pH 8.0, 5 mM Mg[Cl.sub.2], 0.1 mM acetoacetyl CoA.
Amino acids degraded to acetyl CoA or
acetoacetyl CoA are termed ketogenic amino acids since they can give rise to ketone bodies or fatty acids.
The resultant high concentrations of acetyl-CoA and
acetoacetyl CoA would depress the level of ACAT and generate more [beta]-hydroxybutyric acid; high concentrations of acetyl-CoA would also accelerate the Krebs Cycle and produce more ATP, which is stored as phosphocreatine, as a consequence of increased expression of creatine kinase.
(109) Del Rector, F., Blount, W.W., and Leonard, D.R., "Application of the
Acetoacetyl Functionality in Thermoset Coatings," Waterborne and Higher Solids Coatings Symposium, New Orleans, LA (1988).