The A. tumefaciens
LBA4404-ElectroMAX[R] strain carrying the binary vector pCAMBIA1301 (11837 bp) was used.
TaDof1 cassette was co-transformed with pAL154 into AGLI strain of A. tumefaciens
having C58 chromosome background.
A single colony of A. tumefaciens
transformants was cultured in 2 ml of YEP media (10mg/ml Bacto[TM] Tryptone (DIFCO, Detroit, MI), 10 mg/ ml yeast extract (DIFCO, Detroit, MI), and 5mg/ml NaCl (Sigma, St.
This study aimed to assess the possibility of genetically transforming the Brazilian maize BR 451 through A. tumefaciens
, assessing if the high temperatures prevalent when the immature embryos are in the infection phase when h the bacterium raises the transient expression of the UidA reporter gene.
The nonpathogenic strain Agrobacterium radiobacter K84 and the recombinant strain K1026 have been investigated and commercialized for their efficiency as a biological control of A. tumefaciens
for many years [5-10].
strain LBA4404 carrying pCAMBIA1304 was grown on a shaker at 120 rpm and at a temperature of 28[degrees]C for 16 hours to an optical density of 0.8 ([OD.sub.600 nm] = 0.8) in Luria Bertani (LB) medium containing 50 ppm kanamycin.
Material vegetal: se utilizaron embriones maduros de semillas de arveja cultivar Santa Isabel despues de cinco dias de imbibicion en agua, en condiciones de asepsia y con la ayuda de un escalpelo se hicieron cortes transversales a embriones para obtener 32 segmentos de 2 mm de espesor por cepa de A. tumefaciens
a los cuales se colocaron sobre papel filtro humedecido hasta el momento de la infeccion.
were grown at 28degC in Yeast Extract Broth (YEB) medium supplemented with 50 mg/L of streptomycin and 100 mg/L of rifampicin with shaking (220 rpm).
was grown on yeast extract media (YEM) for 48 h at 28[degrees]C.
GM Agrobacterium radiobacter releases a chemical warfare agent bacteriocin (agrocin) against A. tumefaciens
. Bacteriocin prevents the crown gall tumors from forming.
Major stages in the infection by Agrobacterium tumefaciens and the subsequent formation of tumorous galls on the stems of plants involve a series of responses between an individual bacterium (A. tumefaciens
) and mainly dicotyledonous plants (Binns & Thomashow, 1988).
In addition to providing nutrition for the bacterium, the host chemicals spur A. tumefaciens
to produce a responsive protein.
strain was grown in 50 ml of AB liquid media (Hiei et al., 1997) with 40 mg/l rifampicin and 50 mg/l kanamycin, at 28 [+ or -] 1 [degrees]C, in the dark, shaking at 250 rpm.
The efficiency of ATMT in fungi depends upon several factors, including fungal cell type and density, the A. tumefaciens
strain, the time and temperature of cocultivation, and the concentration of the inducer (acetosyringone, AS) during co-cultivation [18-20],