Endophytes were grown for 144h under submerged cultures containing CS; filtrates were then used for the cup plate assay whereby the diameters of enzymatic halos were measured and compared to halos obtained in the positive control, which consisted of a commercial a-amylase
from porcine pancreas.
After harvesting, data were recorded for following morphological and biochemical traits; root length (RL; cm), shoot length (SL; cm), root weight (RW; g), shoot weight (SW; g), a-amylase
Characterization of Alcaligenes faecalis GPA-1 producing thermostable extracellular a-amylase
Studies on the production of a-amylase
by Bacillus subtilis.
and their industrial applications: a review.
Two novel aminooligosaccharides isolated from the culture of Streptomyces coelicoflavus ZG0656 as potent inhibitors of a-amylase
inhibition in the a-amylase
activity = 100 - % relative enzyme activity)
The samples were dispersed in buffer solution and incubated with heat stable a-amylase
at 95-100[degrees]C for 35 min.
from wheat (Triticum aestivum) seeds: its purification, biochemical attributes and active site studies.
Characterization of a-amylase
activity in five species of Mediterranean sparid fishes (Sparidae, Teleostei).
Tenders are invited for Supply of a-Amylase
porcine pancreawse,Pancreatin (from porcine pancrease) (Sigma:A3292-100 g),2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (Sigma:A9941-50TAB),Phe-Pro (Sigma: P6258-1 g)
One unit of a-amylase
is the amount of enzyme that will release 1 mg of reducing sugar in 3 min at 50 C and pH 7.
To prevent the progression of Type 2 DM and for the treatment of prediabetic conditions, one of the most effective therapeutic approaches is the reduction of post-prandial hyperglycemia by inhibiting the carbohydrate hydrolyzing enzymes [alpha]-glucosidase and a-amylase
By acting at random locations along the starch chain a-amylase
breaks down long- chain carbohydrates ultimately yielding maltotriose and maltose from amylase or maltose glucose and limit dextrin" from amylopectin by an endoacting hydrolytic mechanism thereby making it amenable to the action of amyloglucosidase for saccharification to reducing sugar [2 3].
Among the extracts, high level of a-amylase
inhibition was noted in phenolics (17%), while no inhibition was observed in flavonoids and tannin extracts.