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The technique required only one millilitre of urine and found a substantially greater number and different types of microRNAs compared with the standard ultracentrifugation approach.
The sonicated material was centrifuged at 5000 x g to remove the cell debris followed by ultracentrifugation of supernatant at 1, 00,000 x g for 1 hr at 4[degrees]C (Beckman Coulter).
Weisman, "Advanced sorting of single-walled carbon nanotubes by nonlinear density-gradient ultracentrifugation," Nature Nanotechnology, vol.
Pellets formed during ultracentrifugation (bluish gray hemocyanin (HC) pellet) were saved; the supernatant was discarded.
The VAP test uses single vertical spin, inverted rate zonal, and density gradient ultracentrifugation simultaneously to measure concentrations of cholesterol for all five lipoproteins LDL, VLDL, IDL, HDL, Lp(a), and subclasses.
The phage filtrate was further concentrated by ultracentrifugation as per Eyer et al.
Comparison of two homogeneous assays with a precipitation method and an ultracentrifugation method for the measurement of HDL-cholesterol.
These extracellular vesicles can be isolated by ultracentrifugation (21), and it is not known if overlapping can occur between these two different populations of extracellular vesicles.
We are planning a new study with greater patient size according to the suggestion of the author, and we plan to use our formula (CHOLINDEX) in all of the old and new LDL-C measurement formulas (Friedewald, de Cordova CM) and ultracentrifugation followed by beta-quantitation methods.
1) Conventional treatment of lipemia involves ultracentrifugation of specimens at approximately 199 000g to pellet and clarify interfering lipids.
Serum LDL- and HDL--cholesterol determined by ultracentrifugation and HPLC.
To determine how HDL-C and LDL-C particles are affected by testosterone administration to eugonadal men, testosterone was administered as a supraphysiological dose (600 mg/wk) for 3 wk to elderly, obese, eugonadal men before elective hip or knee surgery, and lipids were measured by routine methods and by density gradient ultracentrifugation.
45um), followed by ultracentrifugation at 50,500xg for 120min at 4degC.
Traditional methods to recover exosomes require ultracentrifugation of samples at high centrifugal force (~100,000 x g or more) for at least several hours to pellet the particles.
Technologies addressed include native electrophoresis, affinity chromatography, size-exclusion chromatography, analytical ultracentrifugation, differential scanning calorimetry, light scattering, luminescence, near-infrared spectroscopy, mid-infrared and Fourier transform infrared spectroscopy, Raman spectroscopy, UV-VIS spectroscopy, optical rotatory dispersion and circular dichroism, nuclear magnetic resonance, chemical probes, hydrogen exchange, chemical modification, immunology, and limited proteolysis.
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